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amajLime chromoprotein

Plasmid

#117843

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amajLime

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTarget TMEM97-P2A-mCardinal

Plasmid

#157747

Purpose

Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.

Depositor

Andrew Kruse

Article

Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

Insert

TMEM97 (TMEM97 Human)

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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fwYellow chromoprotein

Plasmid

#117841

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, fwYellow

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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p5E-mpeg1.1

Plasmid

#75023

Purpose

Can be used to drive expression in zebrafish microglia.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

mpeg1.1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pC1 Grx1-roCherry-mito

Plasmid

#141069

Purpose

Fluorescent biosensor for 2GSH/GSSG ratio with mitochondrial localization

Depositor

Dmitry Bilan

Article

Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

Insert

Grx1-roCherry

Tags

dMTS

Expression

Mammalian

Mutation

Changed Alanine 150 to Cysteine, inserted Threoni…

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

Plasmid

#136382

Purpose

CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b (D570A)-TV-T2A-MCP-VPR

Use

CRISPR

Expression

Plant

Mutation

D570A

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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scOrange chromoprotein

Plasmid

#117840

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, scOrange

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pV1524

Plasmid

#111431

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP

Plasmid

#192273

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloning

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin W403A

Plasmid

#227357

Purpose

For bacterial expression of hyperactive parkin mutant.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin W403A (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ223 (dAsCpf1-SRDX)

Plasmid

#86209

Purpose

Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

dAsCpf1

Use

Gateway compatible cpf1 vectors

Tags

NLS and SRDX repressor and NLS

Expression

Plant

Mutation

D908A

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}

Plasmid

#232879

Purpose

Expresses the 5-HT6R coupled to the RGECO1 calcium sensor

Depositor

Paul DeCaen

Article

DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

Insert

5-Hydroxytryptamine Receptor 6 (HTR6 Human)

Use

Lentiviral

Tags

RGECO1.1

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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p221a-GUS

Plasmid

#71263

Purpose

Entry clone containing the GUS enzyme. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

Beta-glucuronidase

Use

Gateway

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBlastR-12xLexAOp-mCherry-MiniWhite

Plasmid

#165917

Purpose

Blasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable LexAOp Response Vector

Use

Synthetic Biology

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+ Grx1-roCherry

Plasmid

#141068

Purpose

Fluorescent biosensor for 2GSH/GSSG ratio

Depositor

Dmitry Bilan

Article

Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

Insert

Grx1-roCherry

Expression

Mammalian

Mutation

Changed Alanine 150 to Cysteine, inserted Threon…

Promoter

CMV, SP6

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-4xTR-TUBE

Plasmid

#110312

Purpose

E. coli expression and purification of 4xTR-TUBE

Depositor

Yasushi Saeki

Article

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

4xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Lb

Plasmid

#86197

Purpose

LbCpf1 Gateway gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

gRNA cloning site

Use

CRISPR

Promoter

Maize ubiquitin 1

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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hKDR

Plasmid

#83436

Purpose

human VEGFR2 with V5 and tGFP tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Cell Physiol. 2018 Aug 1;31

Insert

human VEGFR2 (KDR Human)

Tags

V5 and turboGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19-mAirn-R-loop

Plasmid

#134900

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

mAirn promoter (Airn Mouse)

Use

Synthetic Biology

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-hSNRPN-R-loop

Plasmid

#134902

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

hSNRPN promoter (SNRPN Human)

Use

Synthetic Biology

Promoter

T7

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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