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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,379 results for: Cre

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Showing: 6681 - 6700 of 14379 results

  1. pCAGGS-SARS2-S-G614 (NC-flag)

    Plasmid
    #156419
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
    Depositor
    Hyeryun Choe, Michael Farzan
    Publication
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S protein (S SARS CoV2)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    CMV
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156419-plasmid-map-sequence-id-308855

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  2. pCPP5912

    Plasmid
    #128703
    Purpose
    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone
    Depositor
    Alan Collmer
    Publication
    Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi
    Insert
    shcE-avrE1
    Expression
    Bacterial
    Mutation
    Three synonymous mutations Ala 362 (GCT-GCC), Ala…
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128703-plasmid-map-sequence-id-252488

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  3. IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1

    Plasmid
    #121824
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sa-dCas9/LSD1 (open)
    Use
    Gateway Cloning and Synthetic Biology
    Tags
    NLS
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121824-plasmid-map-sequence-id-237376

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  4. pIMVAd40R

    Plasmid
    #98309
    Purpose
    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;
    Depositor
    Claudia Vickers
    Publication
    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(
    Insert
    ERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1
    Expression
    Yeast
    Available since
    Feb. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    98309-plasmid-map-sequence-id-182601

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  6. Ii-Str_ManII-SBP-tagBFP

    Plasmid
    #65257
    Purpose
    synchronize trafficking of ManII from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ii-Streptavidin and truncated Mannosidase II
    Tags
    Streptavidin Binding Protein (SBP) and tagBFP
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65257-plasmid-map-sequence-id-110465

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  7. Cenp-B INCENP mCherry

    Plasmid
    #45235
    Purpose
    Target INCENP to centromeres via full length CENP-B
    Depositor
    Michael Lampson
    Publication
    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cenp-B, INCENP-Δcen, mCherry
    Tags
    full length CENP-B and mCherry
    Expression
    Mammalian
    Mutation
    CEN box deleted from INCENP (aa1-46)
    Available since
    Sept. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    45235-plasmid-map-sequence-id-84488

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  10. pVenusCfC

    Plasmid
    #128567
    Purpose
    Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.
    Depositor
    Oliver Vugrek
    Publication
    Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus
    Expression
    Mammalian
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    128567-plasmid-map-sequence-id-321691

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  11. pVenusCfN

    Plasmid
    #128566
    Purpose
    Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.
    Depositor
    Oliver Vugrek
    Publication
    Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus
    Expression
    Mammalian
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    128566-plasmid-map-sequence-id-513794

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  15. Sema3e(L)-AP-His

    Plasmid
    #72019
    Purpose
    Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Publication
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sema3e (Sema3e Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72019-plasmid-map-sequence-id-131205

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  16. ST-SBP-EGFP_puromycin

    Plasmid
    #65270
    Purpose
    ST reporter only ( no hook) (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    truncated sialyltransferase (ST6GAL1 Human)
    Tags
    EGFP and Streptavidin Binding Protein (SBP)
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 43 of ST
    Promoter
    CMV
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    65270-plasmid-map-sequence-id-120700

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  18. MSP7-bio

    Plasmid
    #47735
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP7 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP7
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47735-plasmid-map-sequence-id-68747

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  19. pSLIK_Hyg_mTurquoise2_Atg4BC74A

    Plasmid
    #161733
    Purpose
    Doxycycline-inducible expression of Atg4B(C74A) fused with mTurquoise2 to inhibit autophagy
    Depositor
    Alec Kimmelman
    Publication
    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:
    Insert
    Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    mTurquoise2
    Expression
    Mammalian
    Mutation
    220-222 TGC (Cys) is changed to GCA (Ala)
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    161733-plasmid-map-sequence-id-318425

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  20. pCAGGS-SARS2-S-FKO (NC-flag)

    Plasmid
    #159363
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.
    Depositor
    Hyeryun Choe, Michael Farzan
    Publication
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S protein (S SARS-CoV-2)
    Tags
    Flag and Flag
    Expression
    Mammalian
    Mutation
    Furin-cleavage site mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159363-plasmid-map-sequence-id-310910

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Showing: 6681 - 6700 of 14379 results