We narrowed to 14,352 results for: Cas9

Showing: 6701 - 6720 of 14352 results

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pEJS507-pCDest2-noAcr-mTagBFP2-IRES

Plasmid

#85748

Purpose

Expresses mTagBFP2 only; used as a control

Depositor

Erik Sontheimer

Publication

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pX459_HR_Prnp_3

Plasmid

#78623

Purpose

pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequence

Depositor

Walker Jackson

Publication

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HR_Prnp_3 (Prnp Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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sgRNA2_IL1RN

Plasmid

#64142

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA2_IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA4_IL1RN

Plasmid

#64152

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA4_IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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AID-C12*-BE4max

Plasmid

#216729

Purpose

Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and nCas9 (Cas9 with D10A mutation) in mammalian cells.

Depositor

Rahul Kohli

Publication

Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AID-C12*-BE4max (AICDA Human)

Expression

Mammalian

Promoter

CMV

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAGM65905

Plasmid

#153217

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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APq5210

Plasmid

#66086

Purpose

co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF

Depositor

Geraldine Seydoux

Publication

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa4-2

Use

CRISPR

Expression

Worm

Promoter

U6

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
scaffold-linker-U6

Plasmid

#89639

Purpose

construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

Depositor

Wensheng Wei

Publication

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pJAT43

Plasmid

#204299

Purpose

For introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.

Depositor

David Stern

Publication

Stern et al bioRxiv 2023.06.17.545412

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ie1-EGFP

Use

CRISPR

Promoter

ie1

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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vHB178

Plasmid

#193457

Purpose

3xHA endogenous tagging of pandoravirus/mollivirus genes

Depositor

Chantal Abergel

Publication

Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

Insert

3xHA

Use

Pandoravirus/mollivirus expression

Promoter

----

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLPC-mCh-ACTB-P2A-EGFP

Plasmid

#165158

Purpose

Expresses an ACTB gene fragment with P2A, flanked by mCherry and EGFP, in Mammalian cells

Depositor

Taekjip Ha

Publication

Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

Insert

mCherry-ACTB-P2A-EGFP (ACTB Human)

Use

Retroviral

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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LBJJ432

Plasmid

#117508

Purpose

AtEC_enh. promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Publication

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pEC_enh:AATG:BsaI

Expression

Plant

Promoter

EC1.2:EC1.1

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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LBJJ383

Plasmid

#117517

Purpose

PCR template for sgRNA classic with polyT as terminator

Depositor

Jonathan D Jones

Publication

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS235

Plasmid

#73683

Purpose

SapTrap 3-site Destination vector for C-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAP975-1

Plasmid

#99498

Purpose

TEV::linker::tagBFP::3XFlag

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::tagBFP::3XFlag

Expression

Bacterial

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCSW24

Plasmid

#86837

Purpose

bacterial expression of anti-CRISPR protein AcrIIA3

Depositor

Joseph Bondy-Denomy

Publication

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA3

Expression

Bacterial

Promoter

araBAD

Available since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAP997-1

Plasmid

#99500

Purpose

3XFlag::tagRFP::linker::TEV

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagRFP::linker::TEV

Expression

Bacterial

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP546-2

Plasmid

#99476

Purpose

3XFlag::mNeptune::Myc

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::mNeptune::Myc

Expression

Bacterial

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCSW65

Plasmid

#86838

Purpose

bacterial expression of anti-CRISPR protein AcrIIA3

Depositor

Joseph Bondy-Denomy

Publication

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA3

Expression

Bacterial

Promoter

araBAD

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pKb-TnpB2

Plasmid

#215334

Purpose

ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site

Depositor

Kutubuddin Molla

Publication

Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10

Insert

Rice codon optimized ISDra2 TnpB

Tags

Not applicable

Expression

Plant

Promoter

Rice ubiquitin promoter for TnpB and maize ubiqui…

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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