We narrowed to 7,679 results for: URE

Showing: 6701 - 6720 of 7679 results

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Src(KD/YF)-SsrA(Y7I)-mCer3

Plasmid

#221000

Purpose

this is microTag (Y7I mutation in SsrA)-Src(D388R/Y529F) with mCerulean3 at C-terminal

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

Src, SsrA, mCerulean3 (Src Mouse)

Tags

mCerulean3

Expression

Mammalian

Mutation

D388R/Y529F in Src, Y7I in SsrA

Promoter

pCMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT38

Plasmid

#218723

Purpose

Expresses N-terminally EGFP-tagged IFT38 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT38 (CLUAP1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT22

Plasmid

#218720

Purpose

Expresses N-terminally EGFP-tagged IFT22 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT22 (RABL5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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hTspan12(1-305)-1D4_pTT5

Plasmid

#216381

Purpose

Mammalian expression vector to transiently express full-length human Tspan12 with a Rho1D4 tag

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Tetraspanin-12 (TSPAN12 Human)

Tags

Rho1D4

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pJT315 AAV-Rapamycin Inducible-ZNF473KRAB-Citrine

Plasmid

#202064

Purpose

Inducible AAV activator

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-ZNF473KRAB-Citrine

Use

AAV; Aav with reporter

Expression

Mammalian

Mutation

ITR deletion

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-A51P/A56P

Plasmid

#216400

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-A51P/A56P

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51 and 56 to Proline, and deleted…

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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rSirt3 shRNA (pLKO.1 vector)

Plasmid

#216807

Purpose

shRNA against rat Sirt3

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

Insert

Sirt3 (Sirt3 Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-FIP200-V314D-mCherry-Hygro

Plasmid

#210854

Purpose

Lentiviral expression vector encoding FIP200 mutant-mCherry

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

V314D

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-FIP200-V44D-mCherry-Hygro

Plasmid

#210853

Purpose

Lentiviral expression vector encoding FIP200 mutant-mCherry

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

V44D

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-457

Plasmid

#200213

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas1 H25A mutation

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-458

Plasmid

#200214

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas1 E184A mutation

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRAW-459

Plasmid

#200215

Purpose

Strep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

PaCas1, PaCas2-3

Tags

Strep II-TEV on Cas1

Expression

Bacterial

Mutation

Cas1 K214A mutation

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-IntS6 AA 1-400

Plasmid

#195071

Purpose

Expresses FLAG-tagged Drosophila IntS6 amino acids 1-400

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS6 (IntS6 Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-IntS6 AA 1-600

Plasmid

#195072

Purpose

Expresses FLAG-tagged Drosophila IntS6 amino acids 1-600

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS6 (IntS6 Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac1-bullfrog saxiphilin

Plasmid

#140595

Purpose

protein expression

Depositor

Dan Minor

Article

Yen et al Sci Adv. 2019 Jun 19;5(6):eaax2650. doi:

Insert

saxiphilin bullfrog

Tags

GFP-His10

Expression

Insect

Mutation

Deletion of M20 - please see depositor comment

Promoter

polyhedrin promoter

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-B4GALT3mut

Plasmid

#203602

Purpose

Reporter plasmid carrying B4GALT3 3'UTR with mutated miR27b target sequences.

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

beta-1,4-galactosyltransferase 3 (B4GALT3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Seed sequences in miR-27b target sequences were m…

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-MAIP1mut

Plasmid

#203604

Purpose

Reporter plasmid carrying MAIP1 3'UTR with mutated miR27b target sequences

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

matrix AAA peptidase interacting protein 1 (MAIP1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

miR-27b target sequences in the 3'UTR was mu…

Promoter

SV40

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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1BR9-AtWUSSTOP

Plasmid

#202053

Purpose

E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

AtWUS E. Coli Codon Optimized (WUS Mustard Weed)

Tags

6xHis and GFP11

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-miniSdd3-BE4max-BlastR

Plasmid

#204849

Purpose

For cytosine base editing using miniSdd3 in mammalian cells

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd3-nSpCas9(D10A)-UGI

Use

CRISPR

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CMV, SV40, EM7

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-COX2-Q6-R18-G1333C-UGI-Puro

Plasmid

#198845

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX2-Q6-R18 TALEN

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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