We narrowed to 7,272 results for: tet

1. pAAV-syn-FLEX-tTA

   Plasmid

   #178516

   Purpose

   AAV vector for Cre recombinase dependent tetracycline-controlled transactivator (tTA) expression

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-NIP45 wt

   Plasmid

   #210266

   Purpose

   Inducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

   Depositor

   Niels Mailand

   Article

   Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

   Insert

   NIP45 wt siRES (NFATC2IP Synthetic)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   siRES

   Promoter

   CMV/TetO2

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-NIP45 wt

   Plasmid

   #210262

   Purpose

   Inducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

   Depositor

   Niels Mailand

   Article

   Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

   Insert

   NIP45 wt siRES (NFATC2IP Synthetic)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   siRES

   Promoter

   CMV/TetO2

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCOA-Leu-FvPPT1

   Plasmid

   #179923

   Purpose

   Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1

   Depositor

   Jens Laurids Sørensen

   Article

   Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

   Insert

   FvPPT1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for expression in Saccharomyces c…

   Promoter

   Yarrowia lipolytica EXP1

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-SUMO-A'-l2-A'-IRES-EmGFP

   Plasmid

   #209125

   Purpose

   Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l2-A' and EmGFP

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   SUMO-A'-l2-A'-IRES-EmGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDA2ScIfm

   Plasmid

   #193786

   Purpose

   Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   Frame-shifted CI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLTATAA synthetic promoter carrying binding sites…

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNB_EA_R1CiM_4EGFP_R3EGFPi2

   Plasmid

   #185968

   Purpose

   Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

   Depositor

   Sangram Bagh

   Article

   Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

   Insert

   Molecular IMPLY gate
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSJ982

   Plasmid

   #176622

   Purpose

   Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

   Depositor

   Alexander Varshavsky

   Article

   Dong et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

   Insert

   Ub-MPGLW-Fbp1
   

   Use

   Tags

   Ubiquitin N-terminal fusion and triple flag tag C…

   Expression

   Yeast

   Mutation

   Changed first 4 N-terminal amino acid residues of…

   Promoter

   TDH3tc3

   Available since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EC2_2_dCas9_VP64_sgRNA

   Plasmid

   #163707

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VP64+SV40 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-uTEV1-MAVS-mTA2

    Plasmid

    #171000

    Purpose

    HiLITR protease with MAVS [R537A, R538A] C-terminal tmd targeting information (Mitochondria and ER insertion)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[R537A, R538A]
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[R537A, R538A]

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-uTEV1-MAVS-mTA3

    Plasmid

    #171001

    Purpose

    HiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[Y536delinsFIV]

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-uTEV1-MAVS-mTA5

    Plasmid

    #171003

    Purpose

    HiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A]
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[Y536delinsFIVLI, R537A]

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQ132-STU-As

    Plasmid

    #138097

    Purpose

    Golden Gate entry vector for 2nd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYPQ133-STU-As

    Plasmid

    #138100

    Purpose

    Golden Gate entry vector for 3rd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5-FRT-TO-Orf6

    Plasmid

    #157726

    Purpose

    mammalian expression of untagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Orf6 (ORF6 SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pcDNA5-FRT-TO-Nsp15

    Plasmid

    #157720

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp15 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pcDNA5-FRT-TO-Nsp14

    Plasmid

    #157717

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pcDNA5-puro-eGFP-HEC1-M4

    Plasmid

    #114036

    Purpose

    Expression of HEC1 M4 mutant tagged with GFP

    Depositor

    Geert Kops

    Article

    Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

    Insert

    GFP tagged - HEC1 -M4 (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M4 (K146E, R153E, K156E)

    Promoter

    CMV TetO

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-puro-eGFP-HEC1-M2

    Plasmid

    #114035

    Purpose

    Expression of HEC1 M2 mutant tagged with GFP

    Depositor

    Geert Kops

    Article

    Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

    Insert

    GFP tagged - HEC1 -M2 (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M2 (K81E, N87E, K89E)

    Promoter

    CMV TetO

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZS1[TreR-L,LacI-L]

    Plasmid

    #60760

    Purpose

    Contains PI driving expression TreR-L, and PI driving expression of LacI-L

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    TreR-L
    Dimeric LacI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    LacI/GalR repressor chimera. LacI DBD with TreR L…

    Promoter

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge