We narrowed to 13,411 results for: BASE
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Plasmid#119621PurposeLevel 0 Part. CDSDepositorInsertdCas9
UseSynthetic BiologyMutationbp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAT.011
Plasmid#119555PurposeSelf replication Level T acceptor vector with LacZ compatible blue/white screening. Modified SEVA vector, RK2 ori vector.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
KN901: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-KRAB
Plasmid#121830PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7)/KRAB (open)
UseGateway Cloning and Synthetic BiologyTagsNLSAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IR904: pMVP (L3-L2) pA; CMV::eGFP-P2A-TETa-pA
Plasmid#121799PurposepMVP L3-L2 entry plasmid, contains polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven GFP-P2A-TETa downstream of gene of interest.DepositorInsertpolyA + CMV::eGFP-P2A-TETa-polyA
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ901: pMVP (L3-L2) P2A-eGFP + WPRE
Plasmid#121772PurposepMVP L3-L2 entry plasmid, contains eGFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest in lentivirus vectors.DepositorInsertP2A-eGFP + WPRE
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ401: pMVP (L3-L2) P2A-Neo + polyA
Plasmid#121781PurposepMVP L3-L2 entry plasmid, contains Neo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Neo selection marker linked by P2A to gene of interest.DepositorInsertP2A-Neo + polyA
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ601: pMVP (L3-L2) P2A-Zeo + polyA
Plasmid#121783PurposepMVP L3-L2 entry plasmid, contains Zeo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Zeo selection marker linked by P2A to gene of interest.DepositorInsertP2A-Zeo + polyA
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LA601: pMVP (L3-L2) P2A-Puro + WPRE
Plasmid#121785PurposepMVP L3-L2 entry plasmid, contains Puro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to a gene in lentivirus vectorsDepositorInsertP2A-Puro + WPRE
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC-NF8-d1-eGFP
Plasmid#122036PurposeAAV vector, NFkappaB-dependent eGFP expressionDepositorInserteGFP
UseAAVAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB005
Plasmid#119678PurposeCoding sequence nptII from E. coli domesticated into pUPD2 with AATG/GCTT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformationDepositorInsertgeneticin resistance marker
UseSynthetic Biology; Domestication of dna parts for…Available SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB029
Plasmid#119713PurposePromoter paf from Penicillium chrysogenum domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.DepositorInsertPromoter paf
UseSynthetic Biology; Domestication of dna parts for…PromoterPromoter pafAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB030
Plasmid#119714PurposeTerminator paf from P. chrysogenum domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformationDepositorInsertTerminator paf
UseSynthetic Biology; Domestication of dna parts for…Available SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
cycLuc_SbMVs
Plasmid#119209PurposeCircularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)DepositorInsertcyclic luciferase with SbMVp cleavage site
UseLuciferaseTagsAU1 tagExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISP_IS
Plasmid#120425PurposeThis plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.DepositorInsertCRISPR spacers targeting IS1, IS5, IS3
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterconstitutiveAvailable SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti PA-mCit-GW
Plasmid#113456PurposeLentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusionsDepositorTypeEmpty backboneUseLentiviralTagsProteinA-mCitrineExpressionMammalianPromoterhUbCAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti PA-mCit-NL
Plasmid#113452PurposeLentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)DepositorInsertProteinA-mCitrine-NanoLuc
UseLentiviral and LuciferaseExpressionMammalianPromoterhUBbCAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PA-mCit-NL
Plasmid#113444PurposeProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)DepositorInsertProteinA-mCitrine-NanoLuc
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PA-NL
Plasmid#113445PurposeProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)DepositorInsertProteinA-NanoLuc
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PatoL-OG241
Plasmid#72841PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.DepositorInsertPatoL
ExpressionBacterialPromoterPatoLAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-1 (GB1204)
Plasmid#75405PurposeProvides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid partDepositorInsertAtU6-1 promoter
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only