We narrowed to 14,726 results for: EGF

1. pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR

   Plasmid

   #24549

   Depositor

   Thomas Wandless

   Article

   Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

   Insert

   pADH1 yeCherry::YAP1 yeGFP-DHFR (YAP1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   1. Truncated version of ADH700 promoter (SbfI/Spe…

   Available Since

   May 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG-ABEmax-P2A-EGFP-ires-puro

   Plasmid

   #121170

   Purpose

   CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.

   Depositor

   Timo Otonkoski

   Insert

   ABEmax-P2A-EGFP-ires-puro
   

   Use

   CRISPR

   Tags

   P2A-EGFP-ires-puro

   Expression

   Mammalian

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICOZ-SB100X[EF1α-CD19 4-1BB-eGFP]

   Plasmid

   #218343

   Purpose

   Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   CD19 CAR mammalian expression cassette
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. JDW 742 (pDONR221-EGFP-KRAS4B-G12V)

   Plasmid

   #156403

   Purpose

   Gateway middle entry clone encoding EGFP-fused to human KRAS4B with G12V mutation, includes a stop codon

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4B-G12V (KRAS Human)
   

   Use

   Gateway cloning

   Tags

   EGFP

   Mutation

   mutant KRAS4B-G12V

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

   Plasmid

   #182824

   Purpose

   Cre-dependent expression of cytosolic APEX

   Depositor

   Yevgenia Kozorovitskiy

   Article

   Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

   Insert

   APEX.NES-P2A-EGFP
   

   Use

   AAV

   Tags

   EGFP reporter (P2A)

   Promoter

   EF1a

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. phage - ubc - nls - 2xmcp - egfp - BirA*

   Plasmid

   #131132

   Purpose

   BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

   Depositor

   Ralf Jansen

   Article

   Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11

   Insert

   NLS-HA-2xMCP-EGFP-BirA*
   

   Use

   Lentiviral

   Tags

   NLS, HA

   Expression

   Mammalian

   Mutation

   BirA* = BIrA >R118G

   Promoter

   human ubiquitin C (UBC)

   Available Since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSems-leader-mEGFP-MBP-TMD(ALA7)

   Plasmid

   #193051

   Purpose

   Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   ALA7-TMD
   

   Tags

   Ig k-chain leader sequence, MBP, and mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYR69 (X-4:4xUAS-SSA1p-365:yEGFP3-CYC1t)

   Plasmid

   #167355

   Purpose

   Integrative plasmid for promoter-reporter 4xUAS-SSA1p-365:yEGFP3-CYC1t

   Depositor

   Michael Jensen

   Article

   Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

   Insert

   4xUAS-SSA1p-365:yEGFP3-CYC1t
   

   Use

   Unspecified

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

   Plasmid

   #140535

   Purpose

   OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   mAID-EGFP-NLS TOL2
   

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)

    Plasmid

    #191141

    Purpose

    Positive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codon

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587-W:EGFP
    

    Expression

    Mammalian

    Mutation

    UAG>UGG (stop>Trp)

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-EGFL7-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156702

    Purpose

    Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    EGFL7 (EGFL7 Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. NSK024 SFFV-mCherry-SG(p53R248Q.s2)SG-EGFP (FLP-IN)

    Plasmid

    #191169

    Purpose

    Expression of a sensor for p53 mutant R248Q in mammalian cells; mCherry marker, EGFP output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:p53(R248Q).s2:EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKS031 - pCAGGS-3XFLAG-BORIS-eGFP-bpA

    Plasmid

    #156443

    Purpose

    For transient expression of mouse BORIS (CTCFL) tagged with eGFP

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse BORIS (Ctcfl Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDisplay-GRAB-rDA2h-IRES-EGFP-CAAX

    Plasmid

    #208695

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h and a membrane-localized EGFP in mammalian cells

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA2h
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG

    Plasmid

    #86871

    Purpose

    To express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    SF3B6 (SF3B6 Human)
    

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    amino acids 7-29 deletion

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-EGFP barcode-5-SV40 polyA

    Plasmid

    #190869

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode5
    

    Use

    AAV

    Mutation

    WT

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP

    Plasmid

    #153071

    Purpose

    Lentiviral vector expressing human ZC3H12D mutated (D95N)

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    Mutated ZC3H12D (D95N) (ZC3H12D Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed D95 to N, known to abrogate the RNase enz…

    Promoter

    EF1α

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TRE-Gluc-RMA-IRES-EGFP

    Plasmid

    #189628

    Purpose

    Expresses Gluc-RMA and EGFP driven by the Tet Response Element (TRE). Plasmid 2 for recording Fos expression.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    Gaussia luciferase fused to Fc
    IRES-EGFP
    

    Use

    AAV and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Promoter

    IRES and TRE

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin

    Plasmid

    #134863

    Purpose

    Expresses EGFP-tagged endoplasmic reticulum marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    RAMP4 (SERP1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSbi-pur-KSR1-CA3-EGFP (C-KSR)

    Plasmid

    #217768

    Purpose

    Fluorescent reporter for ceramide (for stable mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Sleeping beauty transposase-compatible genome ins…

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400 plus vector-based linker LE…

    Promoter

    EF-1alpha promoter

    Available Since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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