We narrowed to 14,099 results for: cas9

1. CSAC-Pers

   Plasmid

   #168281

   Purpose

   Expresses sgRNA in mammalian cells

   Depositor

   Han Kyoung Choe

   Article

   Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

   Insert

   hU6-sgPer1-3-hU6-sgPer2-1-hU6-sgPer2-2
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   U6, hSyn

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK0_047

   Plasmid

   #123977

   Purpose

   Encodes the Cas9 hCLYBL homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   hCLYBL CAS9 Destination - HygroR
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB3034(X-3 MarkerFree)

   Plasmid

   #73272

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6 SgRNA GAPDH

   Plasmid

   #162736

   Purpose

   Mammalian expression

   Depositor

   Amit Choudhary

   Article

   Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

   Insert

   Spacer sequence for GAPDH
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgRNA1_IL1RN

   Plasmid

   #64140

   Purpose

   Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   IL1RN (IL1RN Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. FASTHDR-Cterm-NanoLuc-Puromycin

   Plasmid

   #167209

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   NanoLuc

   Expression

   Mammalian

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR-U6gRNA-PGKpuro2ABFP

   Plasmid

   #136405

   Purpose

   Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses Puromycin linked to TagBFP.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Mutation

   BbsI restriction site at position 437 was removed…

   Promoter

   U6, PDK

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB2899(X-2 MarkerFree)

   Plasmid

   #73271

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Tol2-LyzC-GFP-U6ac-rac2-guides

   Plasmid

   #168241

   Purpose

   "neutrophil specific GFP with ubiquitous rac2 sgRNAs"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   rac2 sgRNAs
   

   Use

   CRISPR

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB2903(XI-2 MarkerFree)

    Plasmid

    #73275

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHDR-eGFP (pXK-1647)

    Plasmid

    #208824

    Purpose

    The HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDR

    Depositor

    Kun Xu

    Article

    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. ScFv-2ERT2-V

    Plasmid

    #120553

    Purpose

    3rd gen transfer vector. Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP, 2 tandem ERT2 and VP64.

    Depositor

    Yu Wang

    Article

    Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

    Insert

    scFvGCN4, sfGFP, GB1, ERT2, VP64
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA3_IL1RN

    Plasmid

    #64151

    Purpose

    Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA3_IL1RN (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. gh88

    Plasmid

    #106759

    Purpose

    expression of gRNA targeting FUT4

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    FUT4 (FUT4 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB3039(XII-2 MarkerFree)

    Plasmid

    #73279

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-tLuc

    Plasmid

    #107552

    Purpose

    tLuc in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Insert

    Luciferase
    

    Use

    AAV

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgRNA2_IL1RN

    Plasmid

    #64142

    Purpose

    Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA2_IL1RN (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sgRNA4_IL1RN

    Plasmid

    #64152

    Purpose

    Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA4_IL1RN (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX459_HR_Prnp_3

    Plasmid

    #78623

    Purpose

    pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequence

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    HR_Prnp_3 (Prnp Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Tol2-LyzC-GFP-U6ac-cdk2-guides

    Plasmid

    #168250

    Purpose

    "neutrophil specific GFP with ubiquitous cdk2 sgRNAs"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    cdk2 sgRNAs
    

    Use

    CRISPR

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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