We narrowed to 18,457 results for: multi

1. lenti-NLS-miRFP670nano-miRFP670

   Plasmid

   #216131

   Purpose

   Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-ExRai-CKAR(T/A)

   Plasmid

   #118410

   Purpose

   Negative-control mutant for ExRai-CKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-CKAR
   

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3b-8XGliBS:EGFP

   Plasmid

   #84602

   Purpose

   Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

   Depositor

   James Chen

   Article

   Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

   Insert

   eGFP
   

   Expression

   Mammalian

   Promoter

   8X Gli-binding site

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti-ER-mTagBFP2

   Plasmid

   #216127

   Purpose

   Lentiviral vector for expressing mTagBFP2 localizing to the endoplasmic reticulum.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-ProlactinEndoplasmicReticulumSignalSequence-mTagBFP2-KDEL-WPRE
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB-CMPE-ePPEplus

   Plasmid

   #205246

   Purpose

   For plant prime editing in wheat plants

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   Detailed in manuscript

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEY11

   Plasmid

   #191017

   Purpose

   Fluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   Ultrapanneuronal
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20942

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   EF1alpha

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBCW

   Plasmid

   #62658

   Purpose

   Mammalian expression of PhiC31o recombinase from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Insert

   PhiC31
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDA_816

   Plasmid

   #201157

   Purpose

   EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   5x-ALFA tag-dEnAsCas12a-VP64
   

   Use

   CRISPR and Lentiviral

   Mutation

   D908A

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. mTAGBFP2 Peroxisome visual barcode

    Plasmid

    #158668

    Purpose

    tagging clonal populations with Peroxisomal mTAGBFP2 fluorescent protein. Adding Peroxisome localization signal to mTAGBFP2 WC

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mTAGBFP2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Puro-Cas9 donor

    Plasmid

    #58409

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-calpainsensor

    Plasmid

    #36182

    Purpose

    detection of activity of ubiquitous calpains

    Depositor

    Isabelle Richard

    Article

    Stockholm et al J Mol Biol. 2005 Feb 11;346(1):215-22. E

    Insert

    alpha-Fodrin cleavage site (Sptan1 Mouse)
    

    Tags

    eCFP and eYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIG3 (pCMV-IRES-GFP version 3)

    Plasmid

    #78264

    Purpose

    Lentiviral empty cloning vector containing IRES and GFP

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    CMV, lentiviral LTR

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20945

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    hSyn

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti_CMV:Flag-YeNL

    Plasmid

    #208849

    Purpose

    Expresses FLAG epitope-tagged YeNL "Yellow" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    YeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_CMV:Flag-OeNL

    Plasmid

    #208850

    Purpose

    Expresses FLAG epitope-tagged OeNL "Orange" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    OeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGCP123-EbpA_g1

    Plasmid

    #153517

    Purpose

    sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

    Plasmid

    #172067

    Purpose

    Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP11x7
    

    Expression

    Insect

    Available Since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGEL1029

    Plasmid

    #225600

    Purpose

    The STU backbone vector for tomato genome editing is based on Mb3Cas12a-RRR (D172R/N573R/K579R)

    Depositor

    Yong Zhang

    Article

    Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti_CMV:Flag-CeNL

    Plasmid

    #208839

    Purpose

    Expresses FLAG epitope-tagged CeNL "Blue" BRET reporter in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

    Insert

    CeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    1xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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