Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 20,029 results for: cas9 genes

Clear keywords
Showing: 6741 - 6760 of 20029 results

  5. pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP

    Plasmid
    #117140
    Purpose
    Express gRNA vs Dmap1
    Depositor
    Kosuke Yusa
    Insert
    gDmap1 v1 (Dmap1 Synthetic)
    Use
    CRISPR and Lentiviral
    Mutation
    Deleted BbsI site within WPRE, modified BFP
    Promoter
    human U6 promoter, mouse PGK promoter
    Available Since
    April 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    117140-plasmid-map-sequence-id-233768

    Enlarge

  7. pMS73

    Plasmid
    #110629
    Purpose
    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Article
    Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d
    Inserts
    short U6 promoter
    cas9
    tnos terminator
    Use
    CRISPR; Self-replicating in ustilago maydis, conf…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Expression
    Bacterial
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U. maydis hsp70 promoter (Kronstad and Leong, 198…
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    110629-plasmid-map-sequence-id-210665

    Enlarge

  8. pGEM-2t

    Plasmid
    #159752
    Purpose
    CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    sgRNA
    Use
    CRISPR
    Available Since
    Oct. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159752-plasmid-map-sequence-id-313824

    Enlarge

  9. pSc1-DD

    Plasmid
    #80439
    Purpose
    Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Tags
    E. coli dihydrofolate reductase
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available Since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80439-plasmid-map-sequence-id-151455

    Enlarge

  10. pSc1-puro

    Plasmid
    #80438
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin
    Depositor
    Ervin Welker
    Article
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Inserts
    sp Cas9 gRNA
    eGFP
    Puromycin resistance
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV, U6, and hPGK
    Available Since
    Sept. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    80438-plasmid-map-sequence-id-151453

    Enlarge

  12. PEA1-Nuclease-Puro HEK3 CTT ins

    Plasmid
    #171995
    Purpose
    Delivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    HEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171995-plasmid-map-sequence-id-341857

    Enlarge

  14. PEA1-Puro-HEK3 CTT ins

    Plasmid
    #171996
    Purpose
    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171996-plasmid-map-sequence-id-338997

    Enlarge

  15. pLEX307_CR-PDHA2S291D

    Plasmid
    #115200
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S291D (PDHA2 Human)
    Use
    Lentiviral
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available Since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    115200-plasmid-map-sequence-id-232426

    Enlarge

  16. pLEX307_CR-PDHA2S293D

    Plasmid
    #115201
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S293D (PDHA2 Human)
    Use
    Lentiviral
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available Since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    115201-plasmid-map-sequence-id-231792

    Enlarge

  17. pLEX307_CR-PDHA2S291D/S293D

    Plasmid
    #115202
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D/S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S291D/S293D (PDHA2 Human)
    Use
    Lentiviral
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available Since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    115202-plasmid-map-sequence-id-231793

    Enlarge

  19. pMLS256

    Plasmid
    #73715
    Purpose
    SapTrap destination vector for building combined sgRNA expression + repair template vectors
    Depositor
    Erik Jorgensen
    Article
    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.
    Insert
    pU6::sgRNA
    Use
    CRISPR
    Expression
    Worm
    Mutation
    SapI insertion site
    Promoter
    C. elegans U6 snRNA pol III promoter
    Available Since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    73715-plasmid-map-sequence-id-200872

    Enlarge

Showing: 6741 - 6760 of 20029 results