We narrowed to 59,446 results for: cloning

1. pYSD153

   Plasmid

   #212929

   Purpose

   Encodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Expression

   Yeast

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYSD141

   Plasmid

   #212928

   Purpose

   Encodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Expression

   Yeast

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PGL3-MK17

   Plasmid

   #21486

   Depositor

   Shoumo Bhattacharya

   Article

   Leung et al Genomics. 1999 Nov 1. 61(3):307-13.

   Insert

   Cited2 Promoter (CITED2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   July 23, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pAGM28521

   Plasmid

   #105359

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   EC1.2en-EC1.1p fusion promoter
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. YPRCΔ15α1

   Plasmid

   #101712

   Purpose

   GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Type

   Empty backbone

   Use

   Yeast expression

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1/Myc-His(-)-HSulf-2 deltaCC

   Plasmid

   #13006

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   human sulfatase 2 deltaCC (SULF2 Human)
   

   Expression

   Mammalian

   Mutation

   changed Cysteine 88 to Alanine

   Available Since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pQTEV-TPT1

   Plasmid

   #31558

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   tumor protein, translationally-controlled 1 (TPT1 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO GFP DNAJA4

   Plasmid

   #19494

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJA4 (DNAJA4 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-Prom1 SV8

   Plasmid

   #184011

   Purpose

   Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286).

   Depositor

   Peter Stoilov

   Article

   Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

   Insert

   Prom1 (Prom1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Wild type cDNA

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC0.008

    Plasmid

    #119618

    Purpose

    Level 0 Part. CDS

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    eYFP
    

    Use

    Synthetic Biology

    Mutation

    bp 242 T to A

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. YPRCΔ15Ω1

    Plasmid

    #101710

    Purpose

    GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Type

    Empty backbone

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTK_BsmBI_LacZ_Cerulean_Nanotag_91

    Plasmid

    #130539

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    LacZ
    

    Use

    Enhancer cloning nanotag reporter vector

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTK_Citrine_Nanotag_23_neg_Control

    Plasmid

    #130562

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    negative oligo sequence
    

    Use

    Enhancer cloning nanotag reporter vector

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJOG603

    Plasmid

    #105356

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    promotor RPS5a (At3g11940; 1700bp)
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC0.286

    Plasmid

    #119627

    Purpose

    Level 0 Part. CDS

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    AbRChl
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-U6-sgRNA-PGK-puromycin-AflII

    Plasmid

    #106404

    Purpose

    Empty guide RNA cloning vector with an AflII site added from Addgene 41824

    Depositor

    Jason Gertz

    Article

    Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-APIC-Cas9

    Plasmid

    #121657

    Purpose

    gateway cloning destination vector for expressing enhancer driven Cas9

    Depositor

    Chun Han

    Article

    Poe et al Genetics. 2018 Nov 30. pii: genetics.118

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3E-Dusp6DM_S159/197A

    Plasmid

    #67684

    Purpose

    3' entry clone with activated Dusp6 (S159/167A)

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Insert

    dusp6 (dusp6 Zebrafish)
    

    Mutation

    S159A, S197A

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJOG022

    Plasmid

    #105384

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    promotor Zea mays Ubiquitin (1500bp)
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. fADL-1e-flag-linker-p3

    Plasmid

    #139441

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, flag-tag and p3
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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