Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 14,582 results for: eng

Clear keywords
Showing: 6761 - 6780 of 14582 results

  3. pcS-RDG-C1C2

    Plasmid
    #200163
    Purpose
    This plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.
    Depositor
    Masako Harada
    Publication
    Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)
    Inserts
    RDG-C1C2
    C1C2 domain of lactadherin
    Tags
    HA and HIS
    Expression
    Mammalian
    Mutation
    sequence adopted from doi: 10.1371/journal.pone.0…
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    200163-plasmid-map-sequence-id-409210

    Enlarge

  6. pEA02

    Plasmid
    #172193
    Purpose
    Suicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.
    Depositor
    Jean-Luc Pernodet
    Publication
    Santos et al Microorganisms. 2022 Apr 16;10(4). pii:
    Insert
    attL
    Expression
    Bacterial
    Available since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    172193-plasmid-map-sequence-id-338644

    Enlarge

  7. pEA03

    Plasmid
    #172194
    Purpose
    Suicide vector carrying the site attR of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.
    Depositor
    Jean-Luc Pernodet
    Publication
    Santos et al Microorganisms. 2022 Apr 16;10(4). pii:
    Insert
    attR
    Expression
    Bacterial
    Available since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    172194-plasmid-map-sequence-id-338646

    Enlarge

  12. pCJ217

    Plasmid
    #162685
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    Tags
    His
    Expression
    Bacterial
    Mutation
    G489C, S530C; codon optimized for E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162685-plasmid-map-sequence-id-320886

    Enlarge

  15. pSCIP-hTRAC

    Plasmid
    #154094
    Purpose
    Self-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chain
    Depositor
    Scott McComb
    Publication
    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA and BAIT targeting human TRAC locus
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    154094-plasmid-map-sequence-id-307107

    Enlarge

  16. pcDNA3-P4:gs:nls:CIBN

    Plasmid
    #135986
    Purpose
    Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    P4 coiled-coil fused to the CIBN domain
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    135986-plasmid-map-sequence-id-269554

    Enlarge

  18. pHybr_r2a>mA-srt-his

    Plasmid
    #124806
    Purpose
    HDR template to knock-in mouse IgA Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgA producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Publication
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Murine IgA
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124806-plasmid-map-sequence-id-244320

    Enlarge

Showing: 6761 - 6780 of 14582 results