We narrowed to 476 results for: chat

1. pLenti-VQR-P2A-GFP-PGK-Puro

   Plasmid

   #110861

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQR
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-iFlpV

   Plasmid

   #140137

   Purpose

   can be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinase

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Has Service

   AAV PHP.eB and AAV1

   Insert

   iFlpV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMega-MaFRSA

   Plasmid

   #200226

   Purpose

   The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..

   Depositor

   Alanna Schepartz

   Article

   Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

   Inserts

   Pyrrolysyl-tRNA synthetase
   pyrrolysyl-tRNA
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   mutated Asparagine 166 to Alanine and Valine 168 …

   Promoter

   proK-lacO and tacI

   Available since

   June 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-iDreV

   Plasmid

   #140136

   Purpose

   can be used to generate AAV virus that will express light-inducible site-specific iDreV recombinase

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Has Service

   AAV PHP.eB and AAV1

   Insert

   iDreV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-cT3G-cERP2-NR2F2

   Plasmid

   #192900

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of NR2F2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   NR2F2 (NR2F2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERP2-NR4A1

   Plasmid

   #192925

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of NR4A1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   NR4A1 (NR4A1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-HF1RA-P2A-GFP-PGK-Puro

   Plasmid

   #110866

   Purpose

   Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-HF1RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   R661A, Q695A, Q926A, and NLS sequence at the N-te…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-xFNLS-P2A-Puro

   Plasmid

   #110872

   Purpose

   Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xFNLS(3.7)
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-xBE4Gam-P2A-Puro

   Plasmid

   #112676

   Purpose

   Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE4Gam
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PGL4-4X TE Down (GQs) Renilla

    Plasmid

    #70039

    Purpose

    Plasmid for testing 5’UTR TE Down (G-quadruplex) folding elements

    Depositor

    Hans-Guido Wendel

    Article

    Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

    Insert

    4x TE Down (G-quadruplexes) in 5'UTR
    

    Use

    Luciferase

    Tags

    renilla luciferase

    Expression

    Mammalian

    Mutation

    4 repeats of TE down motif (G-quadruplexes) in 5&…

    Promoter

    SV40

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only