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We narrowed to 2,005 results for: CCS

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Showing: 1651 - 1700 of 2005 results

  2. pOUPc-Rh159-HA

    Plasmid
    #179344
    Purpose
    All-in-on "tet-on" 3G lentiviral vector plasmid encoding rhesus cytomegalovirus Rh159 (NK cell evasion protein) with a C-terminal HA tag fused to its cytoplasmic tail
    Depositor
    Jeremy Kamil
    Article
    Siddiquey et al J Virol. 2018 Sep 26;92(20). pii: JVI.00
    Insert
    Rh159
    Use
    Lentiviral; All in one tet-on lentiviral vector (…
    Tags
    HA tag
    Expression
    Mammalian
    Promoter
    tet responsive element 3G
    Availability
    Academic Institutions and Nonprofits only
    179344-plasmid-map-sequence-id-355067

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  3. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118155
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    118155-plasmid-map-sequence-id-243704

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  5. pTBL3440 MTK_PBdest lacO-hU6-BtoApegRNA-LacI-mCherry

    Plasmid
    #254862
    Purpose
    peCHYRON destination vector expressing IPTG-inducible B-to-A pegRNA, LacI, and mCherry. Can be integrated into the genome of mammalian cells with PiggyBac transposase.
    Depositor
    Chang Liu
    Article
    Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d
    Inserts
    lacO_hU6_promoter-peCHYRON_BtoA_pegRNA
    pCAG-LacI-rb_glob_polyA
    pEF1alpha-mCherry-BGH_polyA
    Use
    Piggybac integration
    Expression
    Mammalian
    Available Since
    June 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    254862-plasmid-map-sequence-id-512990

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  6. pTBL3439 MTK_PBdest lacO-hU6-AtoBpegRNA-LacI-mCherry

    Plasmid
    #254861
    Purpose
    peCHYRON destination vector expressing IPTG-inducible A-to-B pegRNA, LacI, and mCherry. Can be integrated into the genome of mammalian cells with PiggyBac transposase.
    Depositor
    Chang Liu
    Article
    Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d
    Inserts
    lacO_hU6_promoter-peCHYRON_AtoB_pegRNA
    pCAG-LacI-rb_glob_polyA
    pEF1alpha-mCherry-BGH_polyA
    Use
    Piggybac integration
    Expression
    Mammalian
    Available Since
    June 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    254861-plasmid-map-sequence-id-512989

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  12. pCR8-CDK12(sgR)

    Plasmid
    #208346
    Purpose
    Gateway cloning entry vector with CDK12 with sgRNA resistant silent mutations. Must be recombined into a DEST vector for expression.
    Depositor
    Peter S Nelson
    Article
    Frank et al eLife 2025
    Insert
    cyclin dependent kinase 12 (CDK12 Human)
    Use
    Gateway: entry vector
    Mutation
    Silent mutations in A88, K90, D92, R94, T715, S71…
    Available Since
    Jan. 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    208346-plasmid-map-sequence-id-415904

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  13. pLenti-sgStuffer-GFP-Puro

    Plasmid
    #208349
    Purpose
    Lentivirus sgRNA backbone with GFP and PuroR. Compatible with golden gate cloning (Esp3i/BsmBI)
    Depositor
    Peter S Nelson
    Article
    Frank et al eLife 2025
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Promoter
    hU6
    Available Since
    Jan. 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    208349-plasmid-map-sequence-id-416750

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  15. FUCGW-DEST

    Plasmid
    #208408
    Purpose
    Lentivirus compatible with LR Gateway cloning for UBC-driven gene expression with CMV-GFP in backbone
    Depositor
    Peter S Nelson
    Article
    Frank et al eLife 2025
    Type
    Empty backbone
    Use
    Lentiviral; Gateway: dest vector
    Promoter
    UBC
    Available Since
    Jan. 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    208408-plasmid-map-sequence-id-416921

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  16. FUCRW-DEST

    Plasmid
    #208409
    Purpose
    Lentivirus compatible with LR Gateway cloning for UBC-driven gene expression with CMV-RFP in backbone
    Depositor
    Peter S Nelson
    Article
    Frank et al eLife 2025
    Type
    Empty backbone
    Use
    Lentiviral; Gateway: dest vector
    Promoter
    UBC
    Available Since
    Jan. 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    208409-plasmid-map-sequence-id-416920

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  28. Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

    Plasmid
    #208974
    Purpose
    Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    B103-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208974-plasmid-map-sequence-id-415835

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  29. Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)

    Plasmid
    #208973
    Purpose
    Recruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208973-plasmid-map-sequence-id-415830

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  30. Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)

    Plasmid
    #208972
    Purpose
    Recruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29(+exo)-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208972-plasmid-map-sequence-id-415827

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  31. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

    Plasmid
    #208971
    Purpose
    Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Phi29-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208971-plasmid-map-sequence-id-415826

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  32. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

    Plasmid
    #208970
    Purpose
    Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Phi29(+exo)-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208970-plasmid-map-sequence-id-415821

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  33. pSEVA251_ Ptrc.x.tetO2::D-HicDH

    Plasmid
    #185456
    Purpose
    CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.
    Depositor
    Wolfgang Kroutil
    Article
    Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.
    Insert
    D-HicDH from Lactobacillus paracasei DSM 20008
    Expression
    Bacterial
    Promoter
    Ptrc.x.tetO2
    Available Since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    185456-plasmid-map-sequence-id-367933

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  34. pX330_ACTB-e1 sgRNA / hSpCas9

    Plasmid
    #172830
    Purpose
    Mammalian expression of a sgRNA targeting the exon 2 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172830-plasmid-map-sequence-id-341086

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  35. pX330_ACTB-e2 sgRNA / hSpCas9

    Plasmid
    #172831
    Purpose
    Mammalian expression of a sgRNA targeting the exon 2 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172831-plasmid-map-sequence-id-341083

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  36. pX330_Camk2a sgRNA / hSpCas9

    Plasmid
    #172839
    Purpose
    Mammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172839-plasmid-map-sequence-id-341073

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  37. pX330_ACTB-i5 sgRNA / hSpCas9

    Plasmid
    #172829
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172829-plasmid-map-sequence-id-340926

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  38. pX330_ACTB-i3 sgRNA / hSpCas9

    Plasmid
    #172827
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172827-plasmid-map-sequence-id-340921

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  39. pX330_ACTB-i2 sgRNA / hSpCas9

    Plasmid
    #172826
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172826-plasmid-map-sequence-id-340920

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  49. Lenti-LucAL

    Plasmid
    #174049
    Purpose
    Expresses mALG8 (ITYTWTRL) and mLAMA4 (VGFNFRTL) antigens as a fusion to luciferase and Cre recombinase
    Depositor
    Tyler Jacks
    Article
    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.
    Insert
    LucAL
    Use
    Cre/Lox, Lentiviral, and Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    ALG8 alanine 506 to threonine; LAMA4 glycine 1254…
    Promoter
    Human Ubiquitin C
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    174049-plasmid-map-sequence-id-345204

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Showing: 1651 - 1700 of 2005 results