We narrowed to 20,390 results for: cel.2

1. pBIG1b_nsp7 (SARS-CoV-2)

   Plasmid

   #169178

   Purpose

   Baculoviral transfer vector to express untagged nsp7 (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp7 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   untagged

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBIG1c_nsp8 (SARS-CoV-2)

   Plasmid

   #169179

   Purpose

   Baculoviral transfer vector to express untagged nsp8 (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp8 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   untagged

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. px335 Mettl14 sgRNA #2

   Plasmid

   #61514

   Purpose

   encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

   Depositor

   Jacob Hanna

   Article

   Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

   Insert

   gccgctcccggatctcctgc
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

   Plasmid

   #172066

   Purpose

   Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   mTagBFP2(11x7)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAc5.1B-EGFP-DmTRF4-2

   Plasmid

   #66204

   Purpose

   expresses EGFP-tagged TRF4-2 in Drosophila cells

   Depositor

   Elisa Izaurralde

   Insert

   TRF4-2 (Trf4-2 Fly)
   

   Use

   Drosophila expression

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Actin 5C

   Available since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAD48-daf-2 RNAi

   Plasmid

   #34834

   Depositor

   Cynthia Kenyon

   Article

   Dillin et al Science. 2002 Oct 25;298(5594):830-4.

   Insert

   daf-2 (daf-2 Nematode)
   

   Use

   RNAi

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pJM601-TRA-2::FLAG

   Plasmid

   #27129

   Depositor

   Ding Xue

   Article

   Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

   Insert

   TRA-2 FLAG (tra-2 Nematode)
   

   Use

   Tags

   3x FLAG

   Expression

   Worm

   Mutation

   Replaced TRA-2 stop codon with KpnI and EcoRI and…

   Promoter

   Available since

   Feb. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJM614-TRA-2::GFP

   Plasmid

   #27128

   Depositor

   Ding Xue

   Article

   Mapes et al Proc Natl Acad Sci U S A. 2010 Oct 19. 1

   Insert

   TRA-2 GFP (tra-2 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   replaced stop codon with AgeI and EcoRI added GFP…

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF1α -NWM-Node 2

   Plasmid

   #154254

   Purpose

   expresses reconstructed New World monkey retroCHMP3 Node 2 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

   Insert

   reconstructed Node 2 of New World monkey retroCHMP3
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. GAL4-mNFAT1(2-144)

    Plasmid

    #11108

    Purpose

    Expresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cells

    Depositor

    Anjana Rao

    Article

    Garcia-Rodriguez et al Eur J Immunol. 2000 Aug . 30(8):2432-6.

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Use

    Tags

    GAL4-DBD

    Expression

    Mammalian

    Mutation

    Contains NFAT1 amino acids 2-144; L78P and P140Q …

    Promoter

    Available since

    April 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCerulean3-Bcl-2-s2193

    Plasmid

    #166750

    Purpose

    Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2 in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

    Depositor

    David Andrews

    Article

    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

    Insert

    Bcl-2 (BCL2 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    Human ferritin

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV6-AN-DDK_UBM1*2* POLI

    Plasmid

    #131232

    Purpose

    Mammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domains

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains inactivating mutations in both ubiquitin…

    Promoter

    CMV enhancer + CMV promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCellFree_KA1_SARS-COV-2_S-frag5

    Plasmid

    #169404

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus C-terminal ectodomain of S protein from the furin cleavag with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    C-term frag. S protein after the furin cleavag (S SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CRISPRv2-sgIl1r1_#2-hygro

    Plasmid

    #231990

    Purpose

    Knockout mouse Il1r1

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    sgRNA with Cas9 and hygromycin resistance (Il1r1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJ204-loxP-100RPU.2-ETN-BGHpA-cHS4-100RPU.2-ETN-lox2272

    Plasmid

    #154834

    Purpose

    Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of etanercept

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Inserts

    ETN
    ETN
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    100RPU.2

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272

    Plasmid

    #154829

    Purpose

    Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of erythropoietin

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Inserts

    coEPO
    coEPO
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized (Cricetulus griseus)

    Promoter

    100RPU.2

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PGK1p-Csy4-pA (Construct 2)

    Plasmid

    #55196

    Purpose

    Expresses Csy4 in Mammalian cells for site-specific RNA processing

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    Csy4/Cas6
    

    Use

    Synthetic Biology

    Tags

    6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1-puro shSlp4-a #2

    Plasmid

    #40070

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    Slp4-a shRNA-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCDNA4 CoV-2 nsp1 3xFLAG

    Plasmid

    #176057

    Purpose

    Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHMTc-FBF-2 (121-632)

    Plasmid

    #218664

    Purpose

    Expression of MBP:6xHIS:FBF-2(121-632)

    Depositor

    Judith Kimble

    Article

    Carrick et al Dev Cell. 2024 Mar 11;59(5):661-675.e7.

    Insert

    fbf-2 (fbf-2 Nematode)
    

    Use

    Tags

    MBP-6xHIS

    Expression

    Bacterial

    Mutation

    amino acids 121-632 only

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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