Addgene

We narrowed to 872 results for: vill

Showing: 661 - 680 of 872 results

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pLX304-PCDH7-b

Plasmid

#108539

Purpose

Expresses human PCDH7 transcript variant b in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant b (PCDH7 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC7-JEDI-2P

Plasmid

#179461

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P under the promoter hsp70 for Drosophila (insect) expression

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-EGFP-PolB-PAMmut-Hygro

Plasmid

#176086

Purpose

EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutation in the PAM site used by PolBKOg1

Promoter

CMV

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pECE-M2-AMPKalpha2 M93G

Plasmid

#31653

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)

Use

Tags

M2

Expression

Mammalian

Mutation

changed Methionine 93 to Glycine

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pLV-GG-hUbC-EBPF2-gRNA-TGFB2

Plasmid

#185556

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting TGFB2

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

TGFB2 gRNAs

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pECE-HA-AMPKalpha2 M93G

Plasmid

#31655

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

changed Methionine 93 to Glycine

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1-HRD1 aa 236-444

Plasmid

#185349

Purpose

RING domain-containing GST fusion protein for ubiquitination assays.

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

HRD1/SYVN (SYVN1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 236-444 only

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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LRG/Rosa26

Plasmid

#105510

Purpose

Lentiviral expression of Rosa26 targeting sgRNA

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Rosa26 (Gt(ROSA)26Sor Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-MFAP2

Plasmid

#185555

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting MFAP2

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

MFAP2 gRNAs

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-SOX4

Plasmid

#185552

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting SOX4

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

SOX4 gRNAs

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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TP53-KO_gRNA_2

Plasmid

#195131

Purpose

TP53-targeting gRNA in the LRG2.1 backbone

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

TP53 KO gRNA 2 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Mm_MyoGEF(WT)

Plasmid

#136325

Purpose

Mammalian expression of mouse MyoGEF-WT with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

PLEKHG6 (Plekhg6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pECE-M2-CDC27 S379A

Plasmid

#31662

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

cell division cycle 27 homolog S379A (CDC27 Human)

Use

Tags

M2

Expression

Mammalian

Mutation

changed Serine 379 to Alanine

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-CDC27 S379A

Plasmid

#31674

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

cell division cycle 27 homolog S379A (CDC27 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

changed Serine 379 to Alanine

Promoter

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only
pHAGE-EF1aL-E690Kmut_ABCA3-GFP

Plasmid

#188549

Purpose

Lentiviral vector allowing for EF1aL-driven constitutive expression of E690K mutant human ABCA3:GFP fusion protein

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

ABCA3:GFP (ABCA3 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

amino acid E690->K690 (ABCA3 gene)

Promoter

EF1aL

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-W308Rmut_ABCA3-GFP

Plasmid

#188550

Purpose

Lentiviral vector allowing for EF1aL-driven constitutive expression of W308R mutant human ABCA3:GFP fusion protein

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

ABCA3 (ABCA3 Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

amino acid W308->R308 (ABCA3 gene)

Promoter

EF1aL

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-Hyg-CMV-BCL9

Plasmid

#202667

Purpose

BCL9 overexpression vector with hygromycin resistance

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

BCL9 transcription coactivator (BCL9 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-gp78 RING

Plasmid

#185353

Purpose

RING domain-containing GST fusion protein for ubiquitination assays.

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 cytoplasmic domain (AMFR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Stop codon after aa 393

Promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-gp78 429-611

Plasmid

#185354

Purpose

GST fusion protein for quantification of gp78 levels

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 (AMFR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 429-611 only

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 AUP1 292-410 5E

Plasmid

#185332

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 292-410 only, with mutations R382E/Q383E/K390E…

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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