We narrowed to 917 results for: vill

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pAAVS1-NC-CRISPRi (Gen3)

Plasmid

#73499

Purpose

Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

dCas9-KRAB

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pW17

Plasmid

#158207

Purpose

2lox landing pad plasmid with mariner transposon and transposase used for CRAGE

Depositor

Yasuo Yoshikuni

Article

Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

Insert

loxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-PSMD4

Plasmid

#202666

Purpose

Tet-ON 3rd generation expression vector with inducible expression of PSMD4 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

proteasome 26S subunit ubiquitin receptor, non-ATPase 4 (PSMD4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-EGFP-PolB-Hygro

Plasmid

#176056

Purpose

EGFP fused to the N-terminus of PolB & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pUC119-gRNA

Plasmid

#52255

Purpose

Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.

Depositor

Jen Sheen

Article

Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

Insert

guide RNA targeting AtPDS3 (PDS3 Mustard Weed)

Use

CRISPR; Plant expression

Promoter

Arabidopsis U6 polymerase III promoter

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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Lenti-MAPK14

Plasmid

#200973

Purpose

a mammalian expression vector to express MAPK14

Depositor

Jason Sheltzer

Article

Bhattacharjee et al Cell Chem Biol. 2023 Oct 19;30(10):1211-

Insert

mitogen-activated protein kinase 14 (MAPK14) (MAPK14 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLX303-PCDH7-a

Plasmid

#108538

Purpose

Expresses human PCDH7 transcript variant a in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant a (PCDH7 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE_HRASV12_Hyg

Plasmid

#195143

Purpose

HRas G12V overexpression vector with hygroymycin resistance

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

hRAS (HRAS Human)

Expression

Mammalian

Mutation

G12V

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLX303-PCDH7-d

Plasmid

#108541

Purpose

Expresses human PCDH7 transcript variant d in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant d (PCDH7 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG-DATDCUE

Plasmid

#185340

Purpose

Assessing domain requirement sof AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

FLAG, 6xHis

Expression

Mammalian

Mutation

deletion of aa 90-337 corresponding to the acyl t…

Available Since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pJFRC7-JEDI-2P

Plasmid

#179461

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P under the promoter hsp70 for Drosophila (insect) expression

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P

Expression

Insect

Promoter

hsp70

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG-DCUE

Plasmid

#185339

Purpose

Assessing domain requirements of AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

FLAG, 6xHis

Expression

Mammalian

Mutation

deletion of aa 293-337 corresponding to the CUE d…

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304-PCDH7-c

Plasmid

#108540

Purpose

Expresses human PCDH7 transcript variant c in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant c (PCDH7 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304-PCDH7-b

Plasmid

#108539

Purpose

Expresses human PCDH7 transcript variant b in mammalian cells

Depositor

Kathryn O’Donnell

Article

Zhou et al Cancer Res. 2017 Jan 1;77(1):187-197. do

Insert

PCDH7 transcript variant b (PCDH7 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pECE-M2-AMPKalpha2 M93G

Plasmid

#31653

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)

Tags

M2

Expression

Mammalian

Mutation

changed Methionine 93 to Glycine

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pECE-HA-AMPKalpha2 M93G

Plasmid

#31655

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

changed Methionine 93 to Glycine

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
LRG/Rosa26

Plasmid

#105510

Purpose

Lentiviral expression of Rosa26 targeting sgRNA

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Rosa26 (Gt(ROSA)26Sor Mouse)

Use

Lentiviral

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-TGFB2

Plasmid

#185556

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting TGFB2

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

TGFB2 gRNAs

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HRD1 aa 236-444

Plasmid

#185349

Purpose

RING domain-containing GST fusion protein for ubiquitination assays.

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

HRD1/SYVN (SYVN1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 236-444 only

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-GG-hUbC-EBPF2-gRNA-MFAP2

Plasmid

#185555

Purpose

Lentiviral backbone with EBFP2 reporter and gRNA targeting MFAP2

Depositor

Andrew Wilson

Article

Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

Insert

MFAP2 gRNAs

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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