We narrowed to 8,279 results for: 11
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Plasmid#112198PurposeThis plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.DepositorTypeEmpty backboneTagsELP-tagExpressionBacterialAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only
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pTSara-N
Plasmid#60721PurposeExpresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORFDepositorInsertResidues 1-179 of T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationOnly amino acids 1-179 of T7 RNAPPromoterPBADAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIhh742bp-Luc
Plasmid#53605PurposeReporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterAvailable SinceJune 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 D444-500
Plasmid#19839DepositorInsertMKL1 D444-500 (MRTFA Human)
Tags3xFLAGExpressionMammalianMutationdeleted amino acids 444-500Available SinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRevTRE MKL1-N100 S449A/T450A/S454A
Plasmid#19849DepositorInsertMKL1-N100 S449A/T450A/S454A (MRTFA Human)
UseRetroviralTags3xFLAGMutationdeleted amino acids 1-100 and changed Serine 449,…Available SinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
mhc2dab:mCherry
Plasmid#68997Purposefor making transgenic fishDepositorAvailable SinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGST1-yVps27-UIM1
Plasmid#44426DepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro human lipin 1-1 shRNA
Plasmid#32019DepositorInsertlipin1 shRNA
UseLentiviral and RNAiExpressionMammalianPromoterU6Available SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-GFP
Plasmid#198589PurposeGateway Entry CloneDepositorInsertGFP
UseGateway CloningMutationNone (wildtype)Available SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BALF2
Plasmid#198578PurposeGateway Entry CloneDepositorAvailable SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
(326) pAAV SV40-ZsGreen U6-gRNA
Plasmid#163020PurposeFluorescent reporter for Sa gRNA (Bsa1 sites)DepositorInsertZs Green
UseAAVExpressionMammalianPromoterSV40Available SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
(364) SB KRAB-SadCas9
Plasmid#163022PurposeSleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)DepositorInsertMammalian codon-optimized SadCas9
UseTransposonTagsKRAB and myc NLSExpressionMammalianPromoterTET-OnAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRAN334
Plasmid#120814PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)DepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms
ExpressionBacterialPromoterPtetAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a - NR
Plasmid#89625PurposeExpresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG inductionDepositorInsertNitrate reductase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGLS3-5xHRE-PHLDA3(wt)
Plasmid#72557PurposeHypoxia induced mutant PHLDA3. Contains the HRE from VEGF sequence (5 repeats) upstream of PHLDA3.DepositorAvailable SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A
Plasmid#69792PurposePebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble - Drosophila guanine nucleotide exchange factor
UseP element-based puast vector for gal4-regulated e…TagsHA tagExpressionInsectMutationEncodes Pbl (NP_729306.1) lacking last 9 amino ac…Promoterhsp70 promoterAvailable SinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDEF3‐mTim1 (no tag)
Plasmid#49206Purposeexpresses mouse Tim1DepositorAvailable SinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3-24p3/LCN2-282kB mut-LUC
Plasmid#46867Purposemurine 24p3 minimal promoter with NF-kB mutation as a luciferase reporterDepositorInsert24p3/lipocalin 2 proximal promoter
UseLuciferaseMutationNF- kB site is mutated (see comments)Promoter24p3 promoter (inactive)Available SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3-24p3/LCN2-282C/EBP mut-LUC
Plasmid#46868Purposemurine 24p3 minimal promoter with C/EBP mutation k as a luciferase reporterDepositorInsert24p3/lipocalin 2 proximal promoter
UseLuciferaseMutationCEBP site is mutated (see comments)Promoter24p3 promoter (inactive)Available SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
hyA3A-BE4max
Plasmid#157943PurposeMammalian expression of hyA3A-BE4maxDepositorInserthyA3A-BE4max
UseCRISPRExpressionMammalianAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only