We narrowed to 19,027 results for: Tec

1. pMM0650

   Plasmid

   #129011

   Purpose

   Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
   

   Expression

   Yeast

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMM0648

   Plasmid

   #129009

   Purpose

   Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   5' URA3 homology-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-homology KanR-ColE1
   

   Expression

   Yeast

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM0651

   Plasmid

   #129012

   Purpose

   Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
   

   Expression

   Yeast

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOCC17

   Plasmid

   #118875

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal tagRFP

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C--NotI-ccdB-AscI-tagRFP-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease and tagRFP

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOCC16

   Plasmid

   #118870

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal EGFP

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C--NotI-ccdB-AscI-EGFP-stop-HindIII cassette
   

   Tags

   EGFP and HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOCC218

   Plasmid

   #118907

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS10, cleavable with 3C protease and N-terminal YBBR tag

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS10-3C-YBBR-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS10, cleavable with 3C protease, YBBR tag

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOCC18

   Plasmid

   #118868

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal tagRFP

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-tagRFP-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease, tagRFP

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC171

   Plasmid

   #118888

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP tag

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-SNAP-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6-MBP, cleavable with 3C protease; SNAP tag

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOCC202

   Plasmid

   #118880

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mKate2, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
   

   Tags

   mKate2, HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOCC139

    Plasmid

    #118872

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and mGFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28b+R3(R112C/T180C)C

    Plasmid

    #108194

    Purpose

    Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA3(R112C/T180C)-P450cam fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28b+3(R112C/T180C)C

    Plasmid

    #108193

    Purpose

    Expresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA3(R112C/T180C)-P450cam fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b+2(L171C)X

    Plasmid

    #108192

    Purpose

    Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA2(L171C)-PdX fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    L171C (PCNA2); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b+R2(L171C)X

    Plasmid

    #108191

    Purpose

    Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA2(L171C)-PdX fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    L171C (PCNA2); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b+1(G108C)X

    Plasmid

    #108189

    Purpose

    Expresses PCNA1(G108C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA1(G108C)-PdX fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C (PCNA1); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. UBQ10:sXVE:ATG-3xHA-S11

    Plasmid

    #108238

    Purpose

    Binary vectors used for the inducible expression of tripartite split-sfGFP components (3xHA-S11 as control) in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Insert

    ATG-3xHA-S11
    

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    UBQ10:sXVE:

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGS_C_3xFLAG

    Plasmid

    #101653

    Purpose

    Expression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cells

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, MCS linker, 3xFLAG tag
    

    Tags

    3xFLAG tag

    Expression

    Insect

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-4T-1 mSesn2 (1190)

    Plasmid

    #66916

    Purpose

    bacterial expression of mouse Sesn2

    Depositor

    Michael Karin, Jun Hee Lee

    Article

    Park et al Nat Commun. 2014 Jun 20;5:4233. doi: 10.

    Insert

    Sesn2 (Sesn2 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSTAP-seq_fly-ssp3

    Plasmid

    #86382

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster ssp3 enhancer
    

    Use

    Stap-seq screening vector

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSTAP-seq_fly-ham

    Plasmid

    #86383

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster ham enhancer
    

    Use

    Stap-seq screening vector

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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