166,461 results

1. pLenti_P2A_Puro

   Plasmid

   #211364

   Purpose

   Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

   Depositor

   Jacques Neefjes

   Article

   Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-BCL6-t2A-BCL2

   Plasmid

   #135305

   Purpose

   Overexpression of multiple human genes (BCL2 and BCL6)

   Depositor

   Daniel Hodson

   Article

   Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

   Inserts

   BCL6 (BCL6 Human)
   BCL2 (BCL2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   LTR

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-luciferase-P2A-Neo

   Plasmid

   #105621

   Purpose

   3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   Luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223-SYK

   Plasmid

   #23907

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   SYK (SYK Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 H2B-mIFP T2A Mpro (wt)

   Plasmid

   #163079

   Purpose

   Expresses wild type Mpro (active) and a near-infrared fluorescent protein mIFP fused to H2B

   Depositor

   Xiaokun Shu

   Insert

   Wild type Mpro of SARS-CoV-2 and H2B-mIFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2 LRP6-eGFP

   Plasmid

   #180143

   Purpose

   to make movies of LRP6 vesicles

   Depositor

   Edward De Robertis

   Article

   Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

   Insert

   Human LRP6 (LRP6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNuc-cis

   Plasmid

   #202796

   Purpose

   RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

   Depositor

   David Edgell

   Article

   Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

   Insert

   TevSpCas9, sgRNA cassette, oriT, CenArsHis
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. mLama1 AAV sgRNA 1, 2, 5

   Plasmid

   #135339

   Purpose

   Expression plasmid of VP64-SadCas9 sgRNA 1, 2 and 3

   Depositor

   Ronald Cohn

   Article

   Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

   Insert

   Lama1 VP64-dCas9 sgRNA Guides
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-HA-ER Y537S

   Plasmid

   #49499

   Purpose

   Expresses HA-tagged ER Y537S mutant in mammalian cells

   Depositor

   Sarat Chandarlapaty

   Article

   Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

   Insert

   ESR1 (ESR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Tyrosine 537 to Serine

   Promoter

   CMV

   Available Since

   Dec. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hsyn-GRAB_DA2h (AAV9)

    Viral Prep

    #140554-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_DA2h (#140554). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_DA2h plasmid DNA. Synapsin-driven expression of high-affinity GRAB_DA2h dopamine sensor in neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Promoter

    Syn

    Tags

    None

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only