We narrowed to 8,392 results for: crispr grnas

Showing: 6801 - 6820 of 8392 results

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pTEE

Plasmid

#159748

Purpose

Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

Depositor

Wolfgang Lukowitz

Article

Angulo et al bioRxiv

Insert

gRNA scaffold

Use

CRISPR

Expression

Plant

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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dCas9-ryt

Plasmid

#246022

Purpose

Expresses dCas9 under control of Hsp70 promoter and gRNAs targeting Lctra, yellow, and RFPex

Depositor

Max Scott

Inserts

dCas9
RFPex

Use

CRISPR

Tags

NLS

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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HH-gRNA-HDV-Shuttle-Vector

Plasmid

#169098

Purpose

Backbone plasmid to generate HH-gRNA-HDV segment

Depositor

Marianne Bronner

Article

Gandhi et al Development. 2021 Mar 9. pii: dev.193565

Insert

HH-gRNAbackbone-HDV

Expression

Mammalian

Promoter

NA

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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JME4393

Plasmid

#129657

Purpose

LYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Article

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

Plasmid

#233036

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

RfxCas13d-compatible gRNA expression cassette and hfCas13d

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pBFC0619

Plasmid

#186690

Purpose

VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJEC581

Plasmid

#174369

Purpose

RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

RFP with PAM rich sequence upstream promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pVH-U

Plasmid

#252743

Purpose

Geminivirus derived-CRISPR/Cas9 plasmid cloning of up to four gRNAs. Encodes GFP and hygromycin resistance.

Depositor

Humberto Prieto

Article

et al Agronomy 2026, 16, 216. https://doi.org/

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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pVK-U

Plasmid

#252744

Purpose

Geminivirus derived-CRISPR/Cas9 plasmid cloning of up to four gRNAs. Encodes GFP and kanamycin resistance.

Depositor

Humberto Prieto

Article

et al Agronomy 2026, 16, 216. https://doi.org/

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

Plasmid

#189633

Purpose

Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro

Plasmid

#189636

Purpose

Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro

Plasmid

#189634

Purpose

Lentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs libraries

Depositor

Manuel Kaulich

Article

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

hU6 Cas9-Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-PXN

Plasmid

#227315

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PXN for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of PXN (PXN Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-CLTC

Plasmid

#227312

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CLTC (CLTC Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-ARL13B

Plasmid

#227276

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of ARL13B for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of ARL13B (ARL13B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-H3C2

Plasmid

#207780

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of H3C2 (H3C2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLKV2-hU6-BbsI-PGK-Puro-TagBFP-WPRE

Plasmid

#137994

Purpose

Backbone for generation of ClonMapper gRNA barcode library for use in 10X feature barcoding

Depositor

Amy Brock

Article

Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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dCas9-tra135

Plasmid

#246023

Purpose

Expresses dCas9 under control of Hsp70 promoter and gRNAs targeting Lucilia cuprina transformer gene

Depositor

Max Scott

Inserts

dCas9
RFPex

Use

CRISPR

Tags

NLS

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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dCas9-tra246

Plasmid

#246024

Purpose

Expresses dCas9 under control of Hsp70 promoter and gRNAs targeting Lucilia cuprina transformer gene

Depositor

Max Scott

Inserts

dCas9
RFPex

Use

CRISPR

Tags

NLS

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aac.4

Plasmid

#158406

Purpose

Express single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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