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sgRNA-msIM83

Plasmid

#251968

Purpose

sgRNA for yeast mtDNA editing, with IM83 aptamer inserted into the major stem-loop of the sgRNA

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

sgRNA-msIM83

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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pBC-Msk1

Plasmid

#251970

Purpose

Production of MSK1 protein. This plasmid is also called Msk1 in the paper.

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

Msk1

Tags

V5-6xHis

Expression

Bacterial

Promoter

T7, lacO

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pBC-tMsk1

Plasmid

#251971

Purpose

Production of truncated MSK1 protein. This plasmid is also called tMsk1 in the paper.

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

tMsk1

Tags

V5-6xHis

Expression

Bacterial

Mutation

deleted aa 1-26

Promoter

T7, lacO

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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MTS-ABE-ARG8

Plasmid

#251976

Purpose

Expression of ABE-ARG8 fusion protein in yeast

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

ABE-ARG8

Use

CRISPR

Tags

MTS

Expression

Yeast

Promoter

GPD

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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NLS-ABE8e-GFP

Plasmid

#251972

Purpose

Yeast nDNA editing with ABE8e

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

ABE8e

Use

CRISPR

Tags

3xNLS-3xFLAG-EGFP and NLS

Expression

Yeast

Promoter

GPD

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pPT24-mtArg8m*-V5

Plasmid

#251978

Purpose

For biolistic transformation to express Arg8m* from mtDNA

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

mtArg8m*

Tags

V5

Expression

Yeast

Mutation

premature stop CAA->TAA, Q79*

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pSC6

Plasmid

#233020

Purpose

Inducible Conjugative Plasmid

Depositor

Bogumil Karas

Article

Jaafar et al Can J Microbiol. 2025 May 6;71:1-9. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA-mito-ApplePy1Lowest

Plasmid

#239077

Purpose

Mammalian expression of mitochondria-targeted lowest affinity red fluorescent pyruvate biosensor ApplePy1Lowest

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

ApplePy1Lowest

Expression

Mammalian

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-mito-ApplePy1Low

Plasmid

#239076

Purpose

Mammalian expression of mitochondria-targeted low affinity red fluorescent pyruvate biosensor ApplePy1Low

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

ApplePy1Low

Expression

Mammalian

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-mito-dApplePy1

Plasmid

#239078

Purpose

Mammalian expression of mitochondria-targeted red fluorescent pyruvate control biosensor dApplePy1

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

dApplePy1

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-hPLEKHA7-Myc

Plasmid

#236538

Purpose

For expression of PLEKHA7 (human) in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al Tissue Barriers. 2014 Jan 1;2(1):e28755.

Insert

PLEKHA7 (PLEKHA7 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAl-pelB-DB21

Plasmid

#226994

Purpose

Express nanobody DB21 in E.coli

Depositor

Ning Zheng

Article

Cao et al Nat Commun. 2022 Feb 10;13(1):815. doi:

Insert

DB21

Tags

His

Expression

Bacterial

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAl-pelB-CA14

Plasmid

#226993

Purpose

Express nanobody CA14 in E.coli

Depositor

Ning Zheng

Article

Cao et al Nat Commun. 2022 Feb 10;13(1):815. doi:

Insert

CA14

Tags

His

Expression

Bacterial

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pTrc-CysGA-Im7 WT

Plasmid

#201081

Purpose

Im7 WT inserted into CysGA at G364 via GS linker

Depositor

Shu Quan

Article

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Im7

Expression

Bacterial

Promoter

pTrc

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTrc-CysGA-Linker only

Plasmid

#201080

Purpose

GS linker inserted into CysGA at G364

Depositor

Shu Quan

Article

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Linker only

Expression

Bacterial

Promoter

pTrc

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-CtDCP1_X

Plasmid

#148006

Purpose

Bacterial Expression of CtDCP1

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

CtDCP1

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH020

Plasmid

#186633

Purpose

contains homology arms for the pilA (NIES39_C03030) locus of Arthrospira platensis

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH021

Plasmid

#186634

Purpose

contains homology arms for the NSI (NIES39_Q01230) locus of Arthrospira platensis

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH022

Plasmid

#186635

Purpose

contains homology arms for the oscillin (NIES39_A01430) locus of Arthrospira platensis

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pYCTK010

Plasmid

#176714

Purpose

The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Kazachstania africana (sender part).

Depositor

Victor Sourjik

Article

Krink et al bioRxiv 2021

Insert

mfalpha1Ka

Use

Synthetic Biology

Mutation

Codon-optimized for expression in S. cerevisiae

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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