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pLVX-BirA-UBE3B-Puro

Plasmid

#107351

Purpose

Expresses UBE3B fused to BirA in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

BirA-UBE3B (UBE3B Human)

Use

Lentiviral

Tags

Biotin ligase

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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hC73S-HA

Plasmid

#111529

Purpose

Expresses human KCNB1 C73S in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

Insert

human C73S-Kv2.1 + HA (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

C73S

Promoter

CMV

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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hC73A-HA

Plasmid

#111526

Purpose

Expresses human KCNB1 C73A in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

Insert

human C73A-Kv2.1 + HA (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

C73A

Promoter

CMV

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMV-TRP1

Plasmid

#65335

Purpose

standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TRP1

Use

Synthetic Biology

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-T-TEF2

Plasmid

#65340

Purpose

construction of TEF2 terminator in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2 terminator

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-P-TDH3

Plasmid

#65336

Purpose

construction of TDH3 promoter in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3 promoter

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-T-ADH1

Plasmid

#65339

Purpose

construction of ADH1 terminator in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ADH1 terminator

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-P-CYC1

Plasmid

#65338

Purpose

construction of CYC1 promoter in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1 promoter

Use

Synthetic Biology

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223 PAK5 P50L

Plasmid

#60533

Purpose

Donor vector for human Pak5 P50L mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

P50L

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 P76T

Plasmid

#60534

Purpose

Donor vector for human Pak5 P76T mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

P76T

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 S312C

Plasmid

#60535

Purpose

Donor vector for human Pak5 S312C mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

S312C

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 V463L

Plasmid

#60536

Purpose

Donor vector for human Pak5 V463L mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

V463L

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 V593I

Plasmid

#60539

Purpose

Donor vector for human Pak5 V593I mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

V593I

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
Cs2+Etv4 S100D

Plasmid

#49094

Purpose

expression of Etv4 S100D mutant

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv4 S100D (etv4 Zebrafish)

Use

Xenopus/avian/zebrafish

Expression

Mammalian

Mutation

S100D

Promoter

CMV

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pHR-SIN-GFP-PTEN-Y16C

Plasmid

#30542

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

GFP-PTEN-Y16C (PTEN Human)

Expression

Mammalian

Mutation

GFP-PTEN-Y16C

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-del(1-25)

Plasmid

#30390

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-del(1-25) (PTEN Human)

Expression

Mammalian

Mutation

del(1-25)

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-del(1-15)

Plasmid

#30389

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-del(1-15) (PTEN Human)

Expression

Mammalian

Mutation

del(1-15)

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pre-associated oBC-minP-GFP-mBC backbone (p043)

Pooled Library

#194098

Purpose

pre-associated oBC-minP-GFP-mBC backbone (p043), can directly be used to deposit library of regulatory elements

Depositor

Jay Shendure

Article

Lalanne et al bioRxiv 2022.12.10.519236

Expression

Bacterial and Mammalian

Use

RNAi

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only
scQers promoter control #1 (low: minimal promoter, p027)

Pooled Library

#217420

Purpose

scQers promoter control #1 (low: minimal promoter, p027)

Depositor

Jay Shendure

Article

Lalanne et al bioRxiv 2022.12.10.519236

Expression

Bacterial and Mammalian

Use

RNAi

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only
scQers promoter control #5 (high: EEF1A1 promoter, p028)

Pooled Library

#217424

Purpose

scQers promoter control #5 (high: EEF1A1 promoter, p028)

Depositor

Jay Shendure

Article

Lalanne et al bioRxiv 2022.12.10.519236

Expression

Bacterial and Mammalian

Use

RNAi

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only