We narrowed to 9,672 results for: Pol
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Plasmid#193719PurposeRapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transportDepositorInserthsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Human, Synthetic)
Tags2xHA-FRBExpressionMammalianMutationhsKIF5B(aa1-560), RIGOR: G234APromoterChicken beta-actinAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mScarlet-Giantin
Plasmid#196872PurposeExpresion of Giantin-fused to mScarlet. Used as Golgi markerDepositorAvailable SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-Med25
Plasmid#64771PurposeMammalian protein expression of flag-tagged Med25DepositorAvailable SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP1B 1-199
Plasmid#115326PurposeExpresses residues 1-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-24DepositorAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
DENV4 Rluc Reporter GND mutant Replicon
Plasmid#118586PurposeFor use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.DepositorInsertDENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
UseYeast-e. col shuttle vector prs424MutationThe conserved GDD in the NS5 polymerase gene muta…Available SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-1-NONO(53-312)
Plasmid#135442PurposeExpresses NONO 53-312 in the second site; for co-expression with His-tagged proteinDepositorInsertNon-POU domain-containing octamer-binding protein (NONO Human)
ExpressionBacterialPromoterT7Available SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_sgCtrl#1
Plasmid#174148PurposeLentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor siteDepositorInsertControl sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Trim46
Plasmid#176402PurposeTRIM46 tagged with GFPDepositorAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMS2280
Plasmid#111901PurposessAAV genome with Ple345 (NEFL MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PH-Btk-mRuby2
Plasmid#124657PurposePI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2DepositorInsertBTK (BTK Human)
TagsmRuby2ExpressionMammalianMutationcontains only the PH domain of Bruton tyrosine ki…Available SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 1-143
Plasmid#108286PurposeExpresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278DepositorAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Trim46
Plasmid#176401PurposeTRIM46 tagged with mCherryDepositorAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMS2273
Plasmid#111899PurposessAAV genome with Ple343 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
A3Ai-Cas9n-UGI-NLS
Plasmid#109425PurposeExpresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.DepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
UseCRISPRExpressionMammalianMutationInsertion of an L1 intron into the coding sequenc…PromoterCMVAvailable SinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-extGluc-mKate-NGFR
Plasmid#104831PurposeextGluc luciferase for invivo Imaging, mKate and NGFR for flourescent detectionDepositorInsertsmKate
NGFR
UseLentiviralExpressionMammalianAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)4x
Plasmid#112058PurposeACTB mRNA tagged with 4 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
TagsRiboglow RNA tagExpressionMammalianMutation4 copies of Riboglow tag after stop codonPromoterCMVAvailable SinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-tdTomato
Plasmid#62516PurposeExpresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing proceduresDepositorInserttdTomato
UseAAV and Synthetic BiologyExpressionMammalianPromoterhUbCAvailable SinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-43Q
Plasmid#84352PurposeExpression of the human Huntingtin Exon1 protein containing 43Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
TagsN-terminal Ssp intein (His-tagged)ExpressionBacterialPromoterT7Available SinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-E988K
Plasmid#173945PurposeBacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)DepositorAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1deltaART
Plasmid#174804PurposeBacterial expression of an inactive PARP1 mutant lacking the ART subdomainDepositorInsertPARP1deltaART (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 785-1014Available SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only