We narrowed to 10,978 results for: T7
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Plasmid#174691PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
TagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cas9-His
Plasmid#98158PurposePurification of Cas9 proteinDepositorInsertNLS-Cas9-NLS
Tags6xHisExpressionBacterialPromoterT7Available SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop A
Plasmid#171779PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23DepositorInsertsfGFP-DogTag Loop A
TagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Val2…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
eSrtA(2A-9) pET29b
Plasmid#75145Purposereprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificityDepositorInsertreprogrammed sortase A variant
Tags6x HisExpressionBacterialMutationS102C A104H E105D K138P K152I N160K K162H T164N K…PromoterT7Available SinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-InPAkt
Plasmid#181914PurposeIndicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.DepositorInsertInPAkt
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mPCSK2
Plasmid#122673PurposeExpresses mouse PCSK2 in mammalian cellsDepositorInsertMus musculus proprotein convertase subtilisin/kexin type 2 (Pcsk2) (Pcsk2 Mouse)
ExpressionMammalianPromoterT7Available SinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30a-AlkB-D135S
Plasmid#79051PurposeExpress mutant AlkB protein (D135S) in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
TagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-EKARev
Plasmid#118474PurposeddRFP-based single-color biosensor for monitoring ERK activity.DepositorInsertRAB-EKARev
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianPromoterCMVAvailable SinceApril 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
eSrtA(4S-9) pET29b
Plasmid#75146Purposereprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificityDepositorInsertreprogrammed sortase A variant
Tags6x HisExpressionBacterialMutationN98D S102C A104V A118T F122A K134R F144L I182V E1…PromoterT7Available SinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG_ToeRep_N09_trigger
Plasmid#132740PurposeT7 RNAP-driven expression of first-generation toehold repressor index 9 trigger RNADepositorInsertToeRep_N09_trigger
ExpressionBacterialAvailable SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-1
Plasmid#163127PurposepLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsUbiquitinExpressionBacterialPromoterT7Available SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHis-hTG2
Plasmid#100719PurposeExpression of human transglutaminase 2 in E.coliDepositorInsertTGM2 (TGM2 Human)
TagsHisExpressionBacterialMutationsilent mutation Arg580 (AGG--CGT) to enhance expr…PromoterT7Available SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
Marathon-RT pentamutant for Split-PE (pEH107)
Plasmid#190110PurposeExpresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)DepositorInsertMarathon-RT
TagsbpNLSExpressionMammalianMutationD14R, N26R, D74R, N116K, N197RPromoterCMVAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-YTHDF2
Plasmid#204406PurposeE. coli expression of full-length YTHDF2 with an N-terminal mCherry tagDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
I48 ELP
Plasmid#68937PurposeElastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residueDepositorInsertI48 ELP
ExpressionBacterialPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
E. coli 16S toehold switch sensor
Plasmid#110698PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertE. coli 16S toehold switch sensor
UseSynthetic BiologyPromoterT7Available SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
modRNAc0-ABE8e-P2A-GFP
Plasmid#178177PurposeIVT template plasmid for making ABE8e-P2A-GFP modRNADepositorInsertABE8e
UseIvt template plasmidTagsP2A-GFPPromoterT7 promoterAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
E. coli mRNA toehold switch sensor
Plasmid#110708PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertE. coli species specific toehold switch sensor
UseSynthetic BiologyPromoterT7Available SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-pmEKAR4
Plasmid#174440PurposePlasma membrane-targeted EKAR4.DepositorInsertpmEKAR4
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-TEV-HaloTag-linker-SpyCatcher3
Plasmid#235150PurposeBacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.DepositorInsertHaloTag linked to SpyCatcher3
TagsHisExpressionBacterialPromoterT7Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only