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HA-MBP-TEVpCS-ADGRB1 CTFdeltaTA-FLAG

Plasmid

#217714

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRB1 (ADGRB1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-932

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF5 CTFdeltaTA-FLAG

Plasmid

#217731

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRF5 (ADGRF5 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-996

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG5 CTFdeltaTA-FLAG

Plasmid

#217736

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG5 (ADGRG5 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-232

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRD2 CTFdeltaTA-FLAG

Plasmid

#217721

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRD2 (ADGRD2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-642

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF4 CTFdeltaTA-FLAG

Plasmid

#217730

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRF4 (ADGRF4 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-390

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF3 CTFdeltaTA-FLAG

Plasmid

#217729

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRF3 (ADGRF3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-758

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG3 CTF-FLAG

Plasmid

#217701

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG3 (ADGRG3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-249

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF1 CTFdeltaTA-FLAG

Plasmid

#217727

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRF1 (ADGRF1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-572

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG3 CTFdeltaTA-FLAG

Plasmid

#217734

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG3 (ADGRG3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-255

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG7 CTFdeltaTA-FLAG

Plasmid

#217738

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG7 (ADGRG7 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-421

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-iBB-GECO1

Plasmid

#194215

Purpose

Far-red fluorescent Ca2+ ion indicator for mammalian cell expression.

Depositor

Jin Zhang

Article

Hashizume et al Protein Sci. 2022 Oct;31(10):e4440. doi:

Insert

iBB-GECO1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT PUR mGSK3AWT

Plasmid

#35159

Depositor

Jim Woodgett

Article

Doble et al Dev Cell. 2007 Jun;12(6):957-71.

Insert

GSK-3α (Gsk3a Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1(+)-mTagBFP2-MRTF-A

Plasmid

#182061

Purpose

Mammalian expression vector encoding myocardin-related transcription factor A tagged with mTagBFP2.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mTagBFP2-MRTF-A (MRTFA Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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PIK3CA-WT (pFastBac)

Plasmid

#16644

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA (PIK3CA Human)

Use

Baculoviral

Tags

His

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_GEM4-Halo-FRB

Plasmid

#197057

Purpose

Expression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_GEM7-Halo-FRB

Plasmid

#197058

Purpose

Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac1 Flag BRG1

Plasmid

#1957

Depositor

Robert Kingston

Article

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BRG1 (SMARCA4 Human)

Tags

flag

Expression

Insect

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pCDNA3 MKK7b1(K149A)

Plasmid

#14674

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7b1(K149A) (Map2k7 Mouse)

Expression

Mammalian

Mutation

Lys-149Ala

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-ER-AKAR4

Plasmid

#64733

Purpose

Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

AKAR4

Tags

Cerulean, N-terminal 27 amino acids from CYP450, …

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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KP501: pMVP (L5-L4) eYFP-P2A

Plasmid

#121714

Purpose

pMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP-P2A (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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