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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,083 results for: pET

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Showing: 6821 - 6840 of 10083 results

  3. pPBbsr2-RaichuEV-Rac1HRasCT

    Plasmid
    #214818
    Purpose
    Encoding a Förster resonance energy transfer (FRET) biosensor for Rac1 activity with cyan and yellow fluorescent protein.
    Depositor
    Michiyuki Matsuda
    Publication
    Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RaichuEV-Rac1HRasCT
    Tags
    HRasCAAX
    Expression
    Mammalian
    Promoter
    CAG promoter
    Available since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    214818-plasmid-map-sequence-id-433014

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  5. pHybr_r2a>mG2a-srt-his

    Plasmid
    #124803
    Purpose
    HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Publication
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Murine IgG2a
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124803-plasmid-map-sequence-id-244318

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  6. HpMscS

    Plasmid
    #78310
    Purpose
    H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag
    Depositor
    Douglas Rees
    Publication
    Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:
    Insert
    Mechanosensitive channel of small conductance
    Tags
    6x His Tag and FLAG Tag
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    78310-plasmid-map-sequence-id-151301

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  8. EKAREN5-gl

    Plasmid
    #225958
    Purpose
    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.
    Depositor
    Toru Hiratsuka
    Publication
    Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.
    Insert
    EKAREN5-gl
    Use
    Lentiviral
    Tags
    nls localization motif
    Expression
    Mammalian
    Promoter
    EF-1-alpha promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    225958-plasmid-map-sequence-id-452543

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  11. pKK-RNAi-nucEGFPmiR-TEV-FLAG

    Plasmid
    #105811
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal FLAG.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-FLAG
    Expression
    Mammalian
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    105811-plasmid-map-sequence-id-204856

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  12. p168-759

    Plasmid
    #62619
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)
    Depositor
    Mattheos Koffas
    Publication
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Promoter
    T7
    Available since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62619-plasmid-map-sequence-id-103510

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  15. FNL Combinatorial Cloning Platform

    Plasmid kit
    #1000000211
    Purpose
    A Frederick National Laboratory (FNL) combinatorial cloning set to generate many tagged expression clones in parallel for protein production, in vivo gene expression, and shRNA and CRISPR delivery.
    Depositor
    Dominic Esposito
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Bacterial Expression, Insect Expression, Mammalian Expression
    Cloning type
    MultiSite Gateway
    Available since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only

  18. pKan cpRBP-FL P53

    Plasmid
    #174504
    Purpose
    Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)
    Depositor
    Stewart Loh
    Publication
    Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do
    Insert
    P53 gene (TP53 Human)
    Tags
    20 amino acid linker containing HRV3C protease si…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174504-plasmid-map-sequence-id-344828

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Showing: 6821 - 6840 of 10083 results