We narrowed to 9,800 results for: CAG

Showing: 6841 - 6860 of 9800 results

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pLKO1-puro-U6-sgRNA-TS4

Plasmid

#69236

Purpose

U6 driven SpCas9 sgRNA expression for TS4 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS4 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCGS652

Plasmid

#62220

Purpose

Mammalian expression TALEN vector with N152, C+18 truncations

Depositor

Daniel Voytas

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Type

Empty backbone

Use

TALEN

Tags

AcV5 and SV40 NLS

Expression

Mammalian

Promoter

miniCaggs

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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Cdk12_intron3_sg2_pX330

Plasmid

#127174

Purpose

sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Availability

Academic Institutions and Nonprofits only

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DHL708

Bacterial Strain

#98417

Purpose

Bacterial Strain with Genotype: MC4100 ∆clpPX

Depositor

Johan Paulsson

Article

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Bacterial Resistance

Streptomycin

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only
GFP-ATG13

Plasmid

#171413

Purpose

For the purifcation of human ULK1 complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ATG13 (ATG13 Synthetic)

Expression

Mammalian

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUB-GFP

Plasmid

#11155

Purpose

Mammalian expression vector for expression of GFP (Ubiquitin C promoter)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

Ubiquitin C promoter (UBC Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2 sgSp1

Plasmid

#174907

Purpose

Second generation sgRNA against Sp1

Depositor

Jane Clifford

Article

Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

Insert

Specificity Protein 1 (SP1 Human)

Expression

Mammalian

Mutation

Missense mutation to mutate PAM sequence

Promoter

EFS-NS

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1 T2 CRIPR in pX330

Plasmid

#72833

Purpose

CRISPR/Cas to target the AAVS1 locus in human cells

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Cas9 and gRNA for targeting the AAVS1 locus in human cells

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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Fyn-Fyn Biosensor

Plasmid

#124655

Purpose

FRET biosensor. Fyn-tagged biosensor targeting to membrane

Depositor

Yingxiao Wang

Article

Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

Insert

Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet

Expression

Mammalian

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgControl

Plasmid

#125836

Purpose

KO control

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

Control

Use

Lentiviral

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pv6_CBX1_2xChromo

Plasmid

#179393

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX1_2xChromo

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pXAT2

Plasmid

#80494

Purpose

AAVS1 sgRNA expression vector

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

AAVS1 sgRNA-T2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pHL-avitag3

Plasmid

#99847

Purpose

mammalian expression with secretive signal and avi tag for biotinylation

Depositor

Edith Yvonne Jones

Article

Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

Insert

ephrin b2 (EPHB2 Human)

Tags

Avi

Expression

Mammalian

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pJET1.2-U6

Plasmid

#173157

Purpose

Single guide RNA cassette under U6 promoter

Depositor

Piotr Ziolkowski

Article

Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

Insert

sgRNA cassette under U6 promoter

Expression

Plant

Promoter

AtU6-26

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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VGluT1-SNAPtag

Plasmid

#107371

Purpose

Synaptic vesicle protein VGluT1 fused to the SNAPtag domain

Depositor

David Perrais

Article

Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

Insert

Vesicular Glutamate transporter 1 (Slc17a7 Rat)

Tags

SNAPtag, 104 aa before C term

Expression

Mammalian

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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plko.1/shMCT4 477

Plasmid

#99598

Purpose

Knockdown of MCT4 expression

Depositor

Eli Bar

Article

Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

Insert

MCT4 (SLC16A3 Human)

Use

Lentiviral

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pHL-FcHis

Plasmid

#99846

Purpose

mammalian expression with secretive signal and FC tag

Depositor

Edith Yvonne Jones

Article

Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

Insert

ephrin b2 (EPHB2 Human)

Tags

Fc_His

Expression

Mammalian

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-FOXP2

Plasmid

#153146

Purpose

Expression of Flag tagged human FOXP2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXP2 (FOXP2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiX2-PURO-shLKB1-Hu

Plasmid

#61242

Purpose

Lentivirus expressing shRNA to human LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

LKB1 (STK11 Human)

Use

Lentiviral

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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pWL502-crRNA

Plasmid

#174383

Purpose

The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

a crRNA targeting the template strand

Use

CRISPR; Archaeal expression

Expression

Bacterial

Promoter

PhaR promoter

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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