We narrowed to 14,582 results for: eng
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Plasmid#254300PurposeCloning backbone for guide RNAs compatible with 10x FLEX and CROPseqDepositorInsertSFFV-Puro-P2A-EGFP-WPRE-hU6-sgRNA
UseCRISPR and LentiviralPromoterSFFVAvailable sinceJune 17, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLex307-UCOE-SFFV-puroR-MCP-MLLx3-HSF1
Plasmid#218558PurposeEncodes SFFV promoter-driven MCP-MLLx3-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistanceDepositorInsertMCP-MLLx3-HSF1
UseLentiviralTagsSV40NLSExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…Available sinceAug. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-4xMS2
Plasmid#212626PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable sinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-eGFP-Puro U6 MMP2 g1
Plasmid#170824PurposePiggyBac Cas13d sgRNA plasmid for MMP2 knockdownDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas13d MMP2 gRNA1
UsePiggybac transposonExpressionMammalianPromoterU6Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG eGFP NLS
Plasmid#170830PurposeDonor plasmid for knocking eGFP NLS into AAVS1 safe harbor locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
UseCRISPR, Synthetic Biology, and TALENTagsNLSExpressionMammalianPromoterCAGAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-Ef1a-SunTag-RPS7-PP7-WPRE
Plasmid#198338PurposePB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loopsDepositorInsertGCN4x24-RPS7-3'UTR-PP7 stem loops x24
UsePiggybacTagsPP7 stem loops and SunTagExpressionMammalianPromoterEf1aAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
SspB-mCherry-DHPH-TIAM1
Plasmid#176104PurposeHeterodimerization with iLID, Rac1 activationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTIAM1 (TIAM1 Human)
ExpressionMammalianAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
(369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)
Plasmid#168190PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system.DepositorInsertHA-nLag6-(EAAAK)4-OGA(544-706)
TagsHA-TagExpressionMammalianPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT25_GalL_BE3
Plasmid#143736PurposeExpresses BE3 in yeast cellsDepositorInsertBE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH-8his (Merlin)
Plasmid#136447PurposeMammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tagDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
TagsShort GGSG linker and 8his tagExpressionMammalianMutationExtracellular domain (a.a. 1-716). Codon-optimize…PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 intron 1 gRNA as2
Plasmid#132394PurposeTargets AAVS1 intron 1, gRNA: AGAACCAGAGCCACATTAAC, MLM3636 backboneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA targeting AAVS1 intron 1 (PPP1R12C Human)
UseCRISPRExpressionMammalianPromoterhU6Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-T2-NLuc
Plasmid#135938PurposeExpresses NanoLuciferase targeted to the mitochondira in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNanoLuciferase
TagsT2 mitochondrial localization sequenceExpressionMammalianPromoterEF1aAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-T2-GpNLuc
Plasmid#135949PurposeExpresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGpNLuc LumiFluor
TagsT2 mitochondrial localization sequenceExpressionMammalianPromoterEF1aAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Col7A1-CRISPR-gRNA#exon2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)
Plasmid#124286PurposesgRNA against human Col7A1DepositorInsertCollagen type VII alpha 1 chain (COL7A1 Human)
UseCRISPRAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Q927P4
Plasmid#103131PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertQ927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-A0LWB3
Plasmid#103070PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertA0LWB3(Cas9 coding gene from Acidothermus cellulolyticus (strain ATCC 43068 / 11B))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
b2-TS5
Plasmid#103878Purposefull-length human b2 with tension sensor at b2_743DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertfull-length human b2 with tension sensor at b2_743 (ITGB2 Human)
Tagstension sensor moduleAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-LIC1 G domain
Plasmid#74602Purposeexpresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherryDepositorInsertfull length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianPromoterSFFVAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-SIN-GFP-PTEN-R11A
Plasmid#30540DepositorAvailable sinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP-Ofd1-Myc-G139S
Plasmid#24581DepositorInsertOfd1 (Ofd1 Mouse)
TagsMyc and eGFPExpressionMammalianMutationGlycine 139 changed to serine (codon GGT to AGT)Available sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only