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pET28a-ClbN-NHis

Plasmid

#51497

Purpose

NRPS module containing CATE domains with N-terminal 6His tag

Depositor

Emily Balskus

Publication

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbN

Tags

6x His tag and T7 tag

Expression

Bacterial

Mutation

contains aa30-1094 of NP_754347.1

Promoter

T7

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAM3110

Plasmid

#40247

Depositor

Susan Golden

Publication

Ditty et al Microbiology. 2005 Aug;151(Pt 8):2605-13

Insert

attP Site

Use

Cyanobacteria cloning vector

Promoter

n/a

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pVRc11_987

Plasmid

#49733

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS11_987

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pMIY-mETV3

Plasmid

#34578

Depositor

Peter Murray

Publication

El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

Insert

Etv3 (Etv3 Mouse)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-AK3L1

Plasmid

#23564

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AK3L1 (AK4 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-RFK

Plasmid

#23698

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RFK (RFK Human)

Use

Gateway Cloning

Mutation

1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-STK17B

Plasmid

#23721

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK17B (STK17B Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pAG416GPD-Cerulean-VPS21

Plasmid

#18842

Depositor

Susan Lindquist

Publication

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

VPS21 (VPS21 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMSCV_FLT3

Plasmid

#223566

Purpose

The plasmid is expressed FLT3 in mammalian cells.

Depositor

Hyun Woo Park

Publication

Shin et al Mol Cancer. 2023 Nov 6;22(1):177. doi: 1

Insert

FLT3 wild type (FLT3 Human)

Use

Retroviral

Expression

Mammalian

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-TAZ-S89A

Plasmid

#213588

Purpose

Doxycycline inducible expression of TAZ-S89A cDNA in mammalian cells

Depositor

Channing Der

Publication

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

2xHA

Expression

Mammalian

Mutation

S89A

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-STOP EF1a-rtTA3

Plasmid

#195882

Purpose

control construct of TRE-VEGFA EF1a-rtTA3

Depositor

Elaine Fuchs

Publication

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

VEGFA (Vegfa Synthetic, Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3GS

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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DDX21

Plasmid

#155510

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX21 (DDX21 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

T232A

Promoter

EF-1a

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

Plasmid

#171795

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

Depositor

Jean Giacomotto

Publication

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Expression

Bacterial

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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THOC5

Plasmid

#156084

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

THOC5 (THOC5 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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DHX58

Plasmid

#155542

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DHX58 (DHX58 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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CPSF4

Plasmid

#155477

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CPSF4 (CPSF4 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMVS-V1

Plasmid

#183094

Purpose

Basic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus

Depositor

Lars Angenent, Bastian Molitor

Publication

Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

Inserts

pME2001
thermostable neomycin phosphotransferase gene

Use

Bacterial propagation and conjugative

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgRB1#1

Plasmid

#174144

Purpose

Lentiviral vector expressing a sgRNA against the human RB1 gene

Depositor

Julien Sage

Publication

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

RB1 (RB1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp3 Mac1 - C2 (UCSF)

Plasmid

#169209

Purpose

Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

Depositor

James S Fraser

Publication

Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

Insert

SARS-CoV-2 Nsp3 Mac1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

July 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PL-CRISPR.EFS.tRFP-gIfngr1-1

Plasmid

#166479

Purpose

Expresses spCas9, tRFP and a gRNA targeting mouse Ifngr1

Depositor

Shuguo Sun

Publication

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Ifngr1 (Ifngr1 Mouse)

Use

Lentiviral

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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