We narrowed to 17,467 results for: SHE
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Plasmid#111535PurposeExpresses human KCNB1 C73M in mammalian cells for imagingDepositorInserthuman C73M-Kv2.1 (KCNB1 Human)
UseTagsEGFPExpressionMammalianMutationC73MPromoterCMVAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Leu-eΔK-Flv
Plasmid#83397Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Arg-eΔK-Flv
Plasmid#83396Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Phe-eΔK-Flv
Plasmid#83395Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVP16-tev-PRT1-C195A
Plasmid#83408Purposebacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationC195A substitutionPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVP16-tev-PRT1-C29A
Plasmid#83407Purposebacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationC29A substitutionPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVP16-tev-PRT1-V28A
Plasmid#83406Purposebacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationV28A substitutionPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA1-gltA2-udhA-zwf
Plasmid#87152PurposegltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertgltA1-gltA2-udhA-zwf guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA1-gltA2-udhA-GapAP1
Plasmid#87151PurposegltA1-gltA2-udhA-GapAP1 targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertgltA1-gltA2-udhA-GapAP1 guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-fabI-gltA1-gltA2-gapAP1
Plasmid#87147PurposefabI-gltA1-gltA2-gapAP1 targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertfabI-gltA1-gltA2-gapAP1 guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJM103: 150bp Hps2 RFP on pCM66T
Plasmid#74744PurposepCM66T with shortened pHps2 promoter driving RFP expression (maxRFP = BBa_E1010)DepositorInsertRFP (BioBrick part number BBa_E1010)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpHps2, trimmed (extracted from Methylovorus gluco…Available sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57-sgDedd-2
Plasmid#74436PurposesgRNA expression vector for mouse Dedd gene targeting intron 4DepositorInsertDedd (Dedd Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK652
Plasmid#72470PurposeProduces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)DepositorInsertacetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationMutated histidine-233 to glycinePromoterT7Available sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK520
Plasmid#72479PurposeEncodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic geneDepositorInsertsformylglycinamidine ribonucleotide synthetase, small subunit
hypothetical protein in purSQL operon
UseGene synthesisTagsHis6ExpressionMutationsynthetic genePromotern/a and noneAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK559
Plasmid#71603PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-Y154F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, tyrosine-…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK562
Plasmid#71604PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Leu/Trp165>Phe mutant (AaPurE1fwf-Y154L)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, tyrosine-…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK604
Plasmid#71610PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe mutant (AaPurE1fww-H59N,Y154F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, histidine…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK664
Plasmid#71613PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-H59N,Y154F)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparagine, tyrosine-154 …PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK666
Plasmid#71615PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparagine, tryptophan-97…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK563
Plasmid#71689PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Ile/Trp165>Phe mutant (AaPurE1fwf-Y154I)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, tyrosine-…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only