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LC801: pMVP (L3-L2) eGFP + WPRE

Plasmid

#121771

Purpose

pMVP L3-L2 entry plasmid, contains eGFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest in lentivirus vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP + WPRE

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LC901: pMVP (L3-L2) mCherry + WPRE

Plasmid

#121773

Purpose

pMVP L3-L2 entry plasmid, contains mCherry + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term mCherry fusion to gene of interest in lentivirus vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry + WPRE

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LD001: pMVP (L3-L2) eYFP + WPRE

Plasmid

#121777

Purpose

pMVP L3-L2 entry plasmid, contains eYFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eYFP fusion to gene of interest in lentivirus vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP + WPRE

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KG901: pMVP (L3-L2) HA epitope tag + polyA

Plasmid

#121749

Purpose

pMVP L3-L2 entry plasmid, contains HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LC701: pMVP (L3-L2) eCFP + polyA

Plasmid

#121767

Purpose

pMVP L3-L2 entry plasmid, contains eCFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IO102: pMVP (R4-R3) eGFP open

Plasmid

#121731

Purpose

pMVP R4-R3 entry plasmid, contains eGFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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HJ601: pMVP (R4-R3) mCherry open

Plasmid

#121733

Purpose

pMVP R4-R3 entry plasmid, contains mCherry (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IO601: pMVP (R4-R3) eCFP open

Plasmid

#121735

Purpose

pMVP R4-R3 entry plasmid, contains eCFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IO401: pMVP (R4-R3) eYFP open

Plasmid

#121737

Purpose

pMVP R4-R3 entry plasmid, contains eYFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KH305: pMVP (R4-R3) βgal open

Plasmid

#121739

Purpose

pMVP R4-R3 entry plasmid, contains βgal (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

βgal (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LF801: pMVP (L1-R5) cTnT promoter

Plasmid

#121696

Purpose

pMVP L1-R5 entry plasmid, contains cTnT promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

cTnT promoter (Cardiomyocyte specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IH001: pMVP (L5-L4) HA epitope tag

Plasmid

#121699

Purpose

pMVP L5-L4 entry plasmid, contains HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IH201: pMVP (L5-L4) 3x HA epitope tag

Plasmid

#121700

Purpose

pMVP L5-L4 entry plasmid, contains 3x HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

3x HA epitope tag (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IX203: pMVP (L5-L4) V5 epitope tag

Plasmid

#121705

Purpose

pMVP L5-L4 entry plasmid, contains V5 epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

V5 epitope tag (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KU801: pMVP (L5-L4) mCherry

Plasmid

#121709

Purpose

pMVP L5-L4 entry plasmid, contains mCherry for 4-component MultiSite Gateway Pro assembly. Allows N-term mCherry fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KU901: pMVP (L5-L4) eCFP

Plasmid

#121711

Purpose

pMVP L5-L4 entry plasmid, contains eCFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eCFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KP401: pMVP (L5-L4) eCFP-P2A

Plasmid

#121712

Purpose

pMVP L5-L4 entry plasmid, contains eCFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eCFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eCFP-P2A (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KV001: pMVP (L5-L4) eYFP

Plasmid

#121713

Purpose

pMVP L5-L4 entry plasmid, contains eYFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eYFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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PB-BE3-NLS

Plasmid

#119030

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Expression

Mammalian

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAL928

Plasmid

#119853

Purpose

precusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427C

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

precursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C

Tags

none

Expression

Bacterial

Mutation

Cysteine 169 to Serine, Cysteine 179 to Serine, C…

Promoter

T7

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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