We narrowed to 21,240 results for: SEC

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pCDH-CAG-NCreIntN-T2A-mTagBFP2

Plasmid

#160500

Purpose

Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

NCreIntN

Use

Cre/Lox and Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ST-SBP-mCherry

Plasmid

#65269

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ST-SBP-mCherry

Plasmid

#65269

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F uGFP

Plasmid

#14558

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F (Y142, Y160, Y174) in DH domain

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pMX Vav1 Y3F uGFP

Plasmid

#14558

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F (Y142, Y160, Y174) in DH domain

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pDragon-empty

Plasmid

#155018

Purpose

Empty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.

Depositor

Alberto Rosello-Diez

Article

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Type

Empty backbone

Use

Mouse Targeting

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pDragon-empty

Plasmid

#155018

Purpose

Empty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.

Depositor

Alberto Rosello-Diez

Article

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Type

Empty backbone

Use

Mouse Targeting

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGLucIPZ

Plasmid

#228703

Purpose

Constitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

Depositor

Thorsten Stiewe

Article

Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

Inserts

Gaussia luciferase
non-silencing shRNA

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGLucIPZ

Plasmid

#228703

Purpose

Constitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

Depositor

Thorsten Stiewe

Article

Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

Inserts

Gaussia luciferase
non-silencing shRNA

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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ST-SBP-mCherry_puromycin

Plasmid

#65271

Purpose

ST reporter only ( no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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ST-SBP-mCherry_puromycin

Plasmid

#65271

Purpose

ST reporter only ( no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV eIF4E W73A IRES GFP

Plasmid

#18762

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E W73A (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

W73A

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
MSCV eIF4E W73A IRES GFP

Plasmid

#18762

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E W73A (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

W73A

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pYSD300

Plasmid

#212930

Purpose

Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

AGA1 (AGA1 Budding Yeast)

Expression

Yeast

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD300

Plasmid

#212930

Purpose

Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

AGA1 (AGA1 Budding Yeast)

Expression

Yeast

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-GFP-SFPQ-RT

Plasmid

#97090

Purpose

Encodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

EGFP-SFPQ-RT (SFPQ Human)

Use

CRISPR

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pUC57-GFP-SFPQ-RT

Plasmid

#97090

Purpose

Encodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

EGFP-SFPQ-RT (SFPQ Human)

Use

CRISPR

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-tdTomato-FlagAkt2

Plasmid

#64832

Purpose

Lentiviral expression of murine Flag-Akt2

Depositor

Eva Gonzalez

Article

Kajno et al Biochem J. 2015 Apr 9.

Insert

Akt2 (Akt2 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pLVX-IRES-tdTomato-FlagAkt2

Plasmid

#64832

Purpose

Lentiviral expression of murine Flag-Akt2

Depositor

Eva Gonzalez

Article

Kajno et al Biochem J. 2015 Apr 9.

Insert

Akt2 (Akt2 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pDR111_Bgal NLS

Plasmid

#188396

Purpose

Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Tags

PhoD secretion peptide and SV40 NLS

Expression

Bacterial

Promoter

Phyper spank

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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