We narrowed to 17,731 results for: She

1. pCHA_SBV_NSs

   Plasmid

   #208938

   Purpose

   Expresses C-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells

   Depositor

   Gerald Barry

   Insert

   NSs
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deleted 47 bp at 5' end and 510 bp from 3&#x…

   Promoter

   CMV

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-Flag-RBM24-95~236

   Plasmid

   #208634

   Purpose

   for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236

   Depositor

   Quanfu Ma

   Insert

   human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   only aa 95~236, other residues not included

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS_MmaPylT_EF1_NES-MmaPylRS WT

   Plasmid

   #174901

   Purpose

   expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG and NES

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGW-Fray

   Plasmid

   #203979

   Purpose

   GFP-Fray under actin promoter for cell culture

   Depositor

   Andreas Jenny

   Insert

   Frayed (fray Fly)
   

   Use

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAMW-WnkAAxV

   Plasmid

   #203980

   Purpose

   Myc6 Wnk under actin promoter for cell culture

   Depositor

   Andreas Jenny

   Insert

   Wnk (Wnk Fly)
   

   Use

   Tags

   6xMyc

   Expression

   Insect

   Mutation

   RFxV mutated to AAxV

   Promoter

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-Cas9-Exo-Blast-BFP

   Plasmid

   #196721

   Purpose

   Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-Exo1-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGB-06-07-Vps34

   Plasmid

   #187989

   Purpose

   Plasmid for the protein expression of Vps34 in a bacterial and insect expression system. Internal_Reference: SMC1323

   Depositor

   Sascha Martens

   Insert

   Vps34 (PIK3C3 Human)
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

   Plasmid

   #185061

   Purpose

   Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445

   Depositor

   Bobby Koeleman

   Insert

   U6:pegRNA:scaffold:PBS+RT template
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p2151 pHAGE-hSPC-TFEB-TagBFP-W

   Plasmid

   #188722

   Purpose

   Lentiviral vector allowing for SFTPC-driven expression of TFEB:tagBFP fusion protein

   Depositor

   Darrell Kotton

   Insert

   TFEB (TFEB Human)
   

   Use

   Lentiviral

   Tags

   tagBFP

   Expression

   Mutation

   Promoter

   hSP-C

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57.1-GFP_mmu-miR-292b-3p_sponge

    Plasmid

    #132622

    Purpose

    Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges

    Depositor

    Constance Ciaudo

    Insert

    stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. mT2A-TagRFP-T-linker-dre-PBD*-FNF

    Plasmid

    #182342

    Purpose

    Recombineering cassette plasmid for T2A-TagRFP-T-linker-dre-PBD*-FNF

    Depositor

    Mario Capecchi

    Insert

    T2A-TagRFP-T-linker-dre-PBD*-FNF
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CNN3A-c007

    Plasmid

    #175457

    Purpose

    Protein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.

    Depositor

    Opher Gileadi

    Insert

    CNN3 (CNN3 Human)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV V5-Eva1b

    Plasmid

    #175158

    Purpose

    Lentiviral expression of V5-tagged mouse Eva1b

    Depositor

    Larry Gerace

    Insert

    Eva1b (Eva1b Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV Cenpv-V5

    Plasmid

    #175125

    Purpose

    Lentiviral expression of mouse Cenpv-V5

    Depositor

    Larry Gerace

    Insert

    Cenpv (Cenpv Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330.orup

    Plasmid

    #110404

    Purpose

    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lentiviral human Citron shRNA 1 GFP

    Plasmid

    #155286

    Purpose

    Lentiviral expression of human CIT shRNA, GFP expression, based on Addgene 12247

    Depositor

    Martin Carroll

    Insert

    Citron (CIT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-hSPC-TFEB(delta30)eGFP-W

    Plasmid

    #126693

    Purpose

    Lineage specific over expression of a mutated TFEB in lung alveolar epithelial cells, where TFEB is missing the first 30 amino acids of the sequence.

    Depositor

    Darrell Kotton

    Insert

    TFEB(delta30) (TFEB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted amino acids 1-30, H32D, Deleted Stop Codo…

    Promoter

    hSPC

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLL1273

    Plasmid

    #129288

    Purpose

    Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211 and alpha-L-Galp_687 as inserts

    Depositor

    Lee Lynd

    Inserts

    alpha-Xylp_1211 (Ga0256695_ 1211)
    alpha-L-Galp_687 (Ga0256695_ 0687)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Clostridium thermocellum DSM1313 Cellobiose Phosp…

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL1274

    Plasmid

    #129385

    Purpose

    Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211 and alpha-L-Araf_996 as inserts

    Depositor

    Lee Lynd

    Inserts

    alpha-Xylp_1211 (Ga0256695_ 1211)
    alpha-L-AraF_996 (Ga0256695_ 0996)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Clostridium thermocellum DSM1313 Cellobiose Phosp…

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Myc var1 K51/52 to NQ

    Plasmid

    #127287

    Purpose

    Expresses Myc with putative Ub site mutated

    Depositor

    Christine Milcarek

    Insert

    Myc (NM_001177352) (Myc Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K51 and 52 to N and Q by Q5 mutagenesis

    Promoter

    CMV

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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