We narrowed to 9,675 results for: BLI

1. pET28a-UBC9

   Plasmid

   #228949

   Purpose

   express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Promoter

   T7-lac

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. DDX3X in pENTR

   Plasmid

   #216572

   Purpose

   gateway cloning donor vector containing DDX3X

   Depositor

   Douglas Allan, Christopher Loewen

   Insert

   DDX3X (DDX3X Human)
   

   Use

   Gateway entry plasmid

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine

   Plasmid

   #50456

   Purpose

   Double floxed Gs-coupled rM3D-IRES-mCitrine under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Insert

   rM3D-IRES-mCitrine (CHRM3 Human)
   

   Use

   AAV

   Tags

   HA

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-GluA1-scFv-mNeon-KDEL

   Plasmid

   #230056

   Purpose

   AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA1 single chain variable fragment (scFv)
   

   Use

   AAV

   Tags

   ER retrieval motif (KDEL) and mNeon

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

   Plasmid

   #66708

   Purpose

   expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

   Depositor

   Charles Gersbach

   Insert

   S.Pyogenes VP64-dCas9-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   VP64

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB660-CYCD3;1

   Plasmid

   #140751

   Purpose

   Inducing plant cells to enter mitosis

   Depositor

   Bo Liu

   Article

   Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

   Insert

   Cyclin D3;1 (CYCD3;1 Mustard Weed)
   

   Tags

   TagRFP

   Expression

   Plant

   Promoter

   CaMV 35 promoter

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-4T1-14-3-3 gamma GST

   Plasmid

   #13280

   Depositor

   Michael Yaffe

   Article

   Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

   Insert

   14-3-3 gamma (Ywhag Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

8. Hs.KRAS4b G12S

   Plasmid

   #83144

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12S mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G12S

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG426-GAL-Alpha-synuclein-E46K

   Plasmid

   #181723

   Purpose

   Galactose inducible expression of Alpha-synuclein-E46K

   Depositor

   Alejandro Chavez

   Insert

   Alpha-synuclein (SNCA Human)
   

   Expression

   Yeast

   Mutation

   E46K

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDF1-mAtf4-HT-puro

    Plasmid

    #101795

    Purpose

    Lentiviral vector containing C-terminally Halo-tag tagged mouse ATF4

    Depositor

    Seiichi Oyadomari

    Insert

    Activating transcription factor 4 (Atf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Halo tag

    Expression

    Mammalian

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Hs.NRAS G12V

    Plasmid

    #83174

    Purpose

    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12V mutation

    Depositor

    Dominic Esposito

    Insert

    neuroblastoma RAS viral oncogene homolog (NRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12V

    Available Since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. JMC044-B12

    Plasmid

    #58752

    Purpose

    bacterial expression of PDB 4NW3

    Depositor

    Cheryl Arrowsmith

    Insert

    PDB:4NW3 (KMT2A Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    T7

    Available Since

    Aug. 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. ALDH18A1

    Plasmid

    #38947

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ALDH18A1 (ALDH18A1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. MS2_SC2_S_Delta

    Plasmid

    #175950

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    Delta (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. PTPN11 in pENTR

    Plasmid

    #216604

    Purpose

    gateway cloning donor vector containing PTPN11

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    Gateway entry plasmid

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRE-T2-IRES-His6-Ubiquitin

    Plasmid

    #107107

    Purpose

    Designed to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.

    Depositor

    Michael E. Wright

    Insert

    human ubiquitin UBB (UBB Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    Tet

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-TREtight-mTagBFP2-N2cG

    Plasmid

    #192838

    Purpose

    plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

    Depositor

    Ian Wickersham

    Insert

    mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TREtight

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-GluA1-scFv-Halo

    Plasmid

    #230055

    Purpose

    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Use

    AAV

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. PE-SUMO CRYAB

    Plasmid

    #217670

    Purpose

    For expression of SUMO tagged human alpha B crystallin

    Depositor

    Larry David

    Insert

    CRYAB (CRYAB Human)
    

    Tags

    6XHis and Smt3

    Expression

    Bacterial

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAD_IPF2.0

    Plasmid

    #163124

    Purpose

    A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

    Depositor

    Julie Biteen

    Article

    Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

    Insert

    IFP2.0
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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