We narrowed to 90,075 results for: Mal;

1. pEF_dCas9-VP64

   Plasmid

   #68417

   Purpose

   Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and VP64

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   human EF1[alpha]

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK2_007

   Plasmid

   #123702

   Purpose

   Encodes human pEF1alpha as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pEF1a
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTubulin-miRFP670nano

   Plasmid

   #127429

   Purpose

   Human alpha-tubulin labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   miRFP670nano-human alpha-tubulin
   

   Tags

   miRFP670nano

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-PpU6P-sgRNA-L3L2

   Plasmid

   #113740

   Purpose

   Gateway entry vector containing attL3 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Promoter

   P. patens U6 promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-PpU6P-sgRNA-L1L4

   Plasmid

   #113736

   Purpose

   Gateway entry vector containing attL1 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Promoter

   P. patens U6 promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-hSyn-jRex-GECO

   Plasmid

   #169259

   Purpose

   AAV transfer plasmid for neuronal expression of jRex-GECO.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   JRex-GECO
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. TagRFP-T-VAPB(1- 218)- eMagB

   Plasmid

   #162255

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   TagRFP-T-VAPB(1- 218)- eMagB
   

   Expression

   Mammalian

   Promoter

   beta-actin promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2_Renilla_luc-60A

   Plasmid

   #200397

   Purpose

   A plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

   Depositor

   Xiao Wang

   Article

   Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

   Insert

   Renilla luciferase
   

   Expression

   Mammalian

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Hip1R-tDimerRFP-N1

   Plasmid

   #27700

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   Hip1R (Hip1r Mouse)
   

   Tags

   tDimer-RFP

   Expression

   Mammalian

   Available Since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA-HA-Nedd4(C744E)/2797

    Plasmid

    #11206

    Depositor

    Allan Weissman

    Article

    Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

    Insert

    Nedd4-1 (Nedd4 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    cysteine 744 mutated to glutamic acid

    Available Since

    March 8, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pMaCTag-P22

    Plasmid

    #120033

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with SNAP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    SNAP
    

    Use

    Pcr template

    Tags

    SNAP

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBT371

    Plasmid

    #125612

    Purpose

    Expresses CDA1-BE4max-NG in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    CDA1-BE4max-NG
    

    Expression

    Mammalian

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti X2 Zeo DEST (749-3)

    Plasmid

    #21562

    Purpose

    3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR insertion, Zeo

    Depositor

    Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG223 PRKD1 3'UTR mut

    Plasmid

    #12042

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    PRKD1 3'UTR mut (PRKD1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pTS1017-Tier1-OTetO7-PCMVmin-SEAP

    Plasmid

    #169586

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Expression

    Mammalian

    Promoter

    TetO7-PCMVmin

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 5HT2A_NB2_1

    Plasmid

    #127690

    Purpose

    Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    5HT2A_NB2
    

    Expression

    Mammalian

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTREX GPR68_NB2_2

    Plasmid

    #127691

    Purpose

    Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    GPR68_NB2_2
    

    Expression

    Mammalian

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTEV5_ RpMatB_1

    Plasmid

    #78205

    Purpose

    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor

    Depositor

    Martin Webb

    Article

    Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7

    Insert

    matB (His6 C106A R286C Q457C K488A)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C106A R286C Q457C K488A

    Promoter

    T7

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pT7-EGFP-C1-HsRRP12_K

    Plasmid

    #146766

    Purpose

    Mammalian Expression of HsRRP12

    Depositor

    Elisa Izaurralde

    Article

    Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

    Insert

    HsRRP12 (RRP12 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. MXS_CMV::PuroR-bGHpA

    Plasmid

    #62439

    Purpose

    MXS_chaining vector with CMV::PuroR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Puromycin with CMV Promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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