We narrowed to 9,675 results for: BLI

1. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

   Plasmid

   #201151

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Inserts

   SadCas9-KRAB
   gRNA-TRE
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Mutation

   gRNA sequence: ATCAGTGATAGAGAACGTATG

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAMK2G

   Plasmid

   #38894

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CAMK2G (CAMK2G Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pRSET-His-APEX2

   Plasmid

   #201471

   Purpose

   Recombinant soluble APEX2 for SLAPSHOT labeling

   Depositor

   Yuh-Nung Jan

   Article

   Tuomivaara et al Commun Biol. 2024 Aug 29;7(1):1060. doi:

   Insert

   APEX2
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV2-CAGIG-LAMP1-mGL

   Plasmid

   #222394

   Purpose

   To express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.

   Depositor

   Pantelis Tsoulfas

   Insert

   LAMP1 and mGreenlantern (LAMP1 Homo Sapiens, Human, Synthetic)
   

   Use

   AAV

   Tags

   LAMP1 and mGreenLantern

   Expression

   Mammalian

   Promoter

   CAG or CAGIG

   Available Since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

   Plasmid

   #159912

   Purpose

   Mutagenesis of Kcnd3

   Depositor

   Larry Zweifel

   Insert

   Kcnd3 (Kcnd3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

   Plasmid

   #105715

   Purpose

   In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.

   Depositor

   Rylan Larsen

   Has Service

   AAV5

   Insert

   synaptophysin-GCaMP6s
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   Ef1a, Human elongation factor-1 alpha

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pScDD2_ERdd

   Plasmid

   #109047

   Purpose

   destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).

   Depositor

   Thomas Wandless

   Insert

   ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
   

   Tags

   yeast enhanced GFP with 8 amino acids TSGRGEGQ li…

   Expression

   Yeast

   Mutation

   3 mutations : E380G, R434G, N532S

   Promoter

   SOD1

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG426-GAL-Alpha-synuclein-H50Q

   Plasmid

   #181724

   Purpose

   Galactose inducible expression of Alpha-synuclein-H50Q

   Depositor

   Alejandro Chavez

   Insert

   Alpha-synuclein (SNCA Human)
   

   Expression

   Yeast

   Mutation

   H50Q

   Available Since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CD44Z-c011

   Plasmid

   #175455

   Purpose

   Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.

   Depositor

   Opher Gileadi

   Insert

   CD44 (CD44 Human)
   

   Tags

   His6

   Expression

   Mammalian

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-blast shKGA

    Plasmid

    #110420

    Purpose

    shKGA, silence glutaminase KGA isoform, blasticidin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 (RNA Pol III)

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p-dCas9-p300-Hygro

    Plasmid

    #104411

    Purpose

    transient expression of dCas9-p300 fusion protein

    Depositor

    Stephan Beck

    Insert

    p300 (EP300 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. DOT1L

    Plasmid

    #36196

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DOT1L (GeneScript Synthesized) (DOT1L Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acid residues 1-420

    Available Since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

13. DOT1L

    Plasmid

    #40736

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DOT1L (DOT1L Human)
    

    Tags

    6xHis TEV

    Expression

    Bacterial

    Available Since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. Hs.KRAS4b G12R

    Plasmid

    #83143

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12R mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12R

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. saposin B^W

    Plasmid

    #161759

    Purpose

    Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

    Depositor

    Sam Gellman

    Article

    Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

    Insert

    saposin B R38_W
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    changed Arginine 38 to Tryptophan

    Promoter

    T7

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBabe-Kras G12V-T35S

    Plasmid

    #58900

    Purpose

    Expresses human Kras with G12V and T35S mutations

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12V and T35S mutations (effector binding mutant)

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

17. pTP-AAV-ChATen-mCherry

    Plasmid

    #193741

    Purpose

    This AAV vector contains a ChAT enhancer that drives mCherry expression in spinal cord skeletal motor neurons. Specificity of vector not characterized outside the mouse spinal cord.

    Depositor

    Hynek Wichterle

    Insert

    ChAT enhancer, mini promoter, synthetic intron, mCherry
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Promoter

    1000 bp ChAT enhancer with a 32 bp minimal promot…

    Available Since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEC2735 (pSpy0C2)

    Plasmid

    #220279

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. p413TEF1-ATR1(pYL573)

    Plasmid

    #178288

    Purpose

    Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    ATR1
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid

    #174091

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Promoter

    pCMV and T7

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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