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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,417 results for: GRN;

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Showing: 6941 - 6960 of 16417 results

  7. pUDP002

    Plasmid
    #103872
    Purpose
    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Inserts
    hph
    Spcas9 D147Y P411T
    Use
    CRISPR
    Tags
    NLS
    Expression
    Yeast
    Mutation
    D147Y P411T
    Promoter
    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103872-plasmid-map-sequence-id-199260

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  8. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid
    #127668
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a and hU6
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127668-plasmid-map-sequence-id-247046

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  10. PEA1-Nuclease-Puro HEK3 CTT ins

    Plasmid
    #171995
    Purpose
    Delivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    HEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171995-plasmid-map-sequence-id-341857

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  11. Adeno FT

    Plasmid
    #101824
    Purpose
    Adenovirus for the expression of gRNAs targeting intron 17 of murine Fgfr3 and intron 6 of murine Tacc3
    Depositor
    Andrea Ventura
    Article
    Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10
    Insert
    U6_sgRNA(Fgfr3)_U6_sgRNA(Tacc3)_CBh_FLAG-Cas9 (Fgfr3, Tacc3 Mouse)
    Use
    Adenoviral
    Tags
    FLAG-Cas9
    Promoter
    U6 and CBh
    Available Since
    Nov. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  12. pMZ379

    Plasmid
    #74215
    Purpose
    Combined sgRNA/Cas9 vector with NatMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available Since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74215-plasmid-map-sequence-id-139305

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  13. pDECKO_GFP_AAVS1_186

    Plasmid
    #159091
    Purpose
    targeting, non-essential locus control
    Depositor
    Rory Johnson
    Insert
    gRNA
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    159091-plasmid-map-sequence-id-308784

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  14. pDECKO_mCherry_AAVS1

    Plasmid
    #141212
    Purpose
    pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control
    Depositor
    Rory Johnson
    Insert
    gRNA
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    July 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    141212-plasmid-map-sequence-id-299605

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  15. pMZ377

    Plasmid
    #74214
    Purpose
    Combined sgRNA/Cas9 vector with LEU2 marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available Since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74214-plasmid-map-sequence-id-141045

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  16. LentiGuide-Hygro

    Plasmid
    #139462
    Purpose
    Lentiviral vector with gRNA scaffold and hygromycin selectable marker
    Depositor
    Caroline Goujon
    Insert
    no sgRNA inserted; resistance gene: hygroR
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a / U6
    Available Since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139462-plasmid-map-sequence-id-308474

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  17. pMZ376

    Plasmid
    #74213
    Purpose
    Combined sgRNA/Cas9 vector with KanMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available Since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74213-plasmid-map-sequence-id-139302

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  18. pSpCas9(BB)-2A-Hygro

    Plasmid
    #127763
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127763-plasmid-map-sequence-id-247560

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  19. pCFD5_w

    Plasmid
    #112645
    Purpose
    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.
    Depositor
    Michael Boutros
    Insert
    U6:3-tRNA-sgRNA expression cassette
    Expression
    Insect
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112645-plasmid-map-sequence-id-217990

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  20. pSB700-ACTC1-Puro

    Plasmid
    #128324
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human ACTC1
    Expression
    Mammalian
    Available Since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128324-plasmid-map-sequence-id-254244

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Showing: 6941 - 6960 of 16417 results