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pcDNA.5.1-FHS-TNRC6A-FL-S1332D/S1346D

Plasmid

#215912

Purpose

Expression of full-length TNRC6A with S1332D/S1346D mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

Full length of TNRC6A, S1332D, S1346D

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-FL-S1332A/S1346A

Plasmid

#215913

Purpose

Expression of full-length TNRC6A with S1332A/S1346A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

Full length of TNRC6A, S1332A, S1346A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-FL-S1616D/S1691D

Plasmid

#215914

Purpose

Expression of full-length TNRC6A with S1616D/S1691D mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

Full length of TNRC6A, S1616D, S1691D

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-FL-S1616A/S1691A

Plasmid

#215915

Purpose

Expression of full-length TNRC6A with S1616A/S1691A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

Full length of TNRC6A, S1616A, S1691A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_WT&Mut-CM

Plasmid

#215920

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

one complete-matched (CM) sequence of siBRAF_WT&Mut (BRAF Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_Mut1-CM

Plasmid

#215922

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

one complete-matched (CM) sequence of siBRAF_ Mut1 (BRAF Human)

Expression

Mammalian

Mutation

BRAF

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_Mut2-CM

Plasmid

#215923

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

one complete-matched (CM) sequence of siBRAF_ Mut2 (BRAF Human)

Expression

Mammalian

Mutation

BRAF

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A/S1346A

Plasmid

#215902

Purpose

Expression of TNRC6A-CIM1 domain with S1332A/S1346A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1528, S1332A/S1346A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D

Plasmid

#215906

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332D/S1346D mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332D, S1346D

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A

Plasmid

#215907

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332A, S1346A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA.5.1-FHS-TNRC6A-SD-S1616D/S1691D

Plasmid

#215908

Purpose

Expression of TNRC6A-silencing domain (SD) with S1616D/S1691D mutation

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

contain aa1224-1709, S1616D, S1691D

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-FMR1-dCGG0

Plasmid

#222965

Purpose

To create 0CGG FMR1 cell line

Depositor

Xinyu Zhao

Article

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

FMR1 homologous template no CGG repeats (FMR1 Human)

Use

CRISPR

Mutation

CGG repeats deleted from 5' UTR

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1306-AAV-EFSNC-dSaCas9-HP1aNH(115-177)

Plasmid

#223151

Purpose

Expression of truncated HP1a with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 115-177

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1296-AAV-EFSNC-dCjCas9-HP1aNH(115-177)

Plasmid

#223141

Purpose

Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

HP1a (CBX5 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 115-177

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Nanos (HK65)

Plasmid

#206468

Purpose

Nanos expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Nanos (nos Fly)

Tags

PH mcherry

Expression

Insect

Mutation

M24E

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Bam M24E (HK88)

Plasmid

#206467

Purpose

Bam expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Bam (bam Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-mCherry-Nanos isoform B (JM154)

Plasmid

#206447

Purpose

Nanos expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Nanos (nos Fly)

Tags

mCherry

Expression

Insect

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-mEGFP-CCR4 isoform A (HK52)

Plasmid

#206439

Purpose

CCR4 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

CCR4 (twin Fly)

Tags

mEGFP

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 241-387 (DR) (HK74)

Plasmid

#206462

Purpose

Oskar 240-387 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 240-387 (osk Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Bam isoform A (HK62)

Plasmid

#206466

Purpose

Bam expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Bam (bam Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-CCR4 isoform A-mCherry-PH (EB7)

Plasmid

#206473

Purpose

CCR4 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

CCR4 (twin Fly)

Tags

mcherry PH

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-OST4-mCherry-CAF1 isoform A (HK184)

Plasmid

#206476

Purpose

CAF1 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

CAF1 (Pop2 Fly)

Tags

OST4 mcherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_DelCTerm_1-223AA

Plasmid

#221488

Purpose

NKX2-1 C-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 224-371AA C-terminal domain

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pXPR_003.gEZH2

Plasmid

#220318

Purpose

Expresses guide RNA for CRISPR/Cas9 knockout of EZH2 for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 gRNA (EZH2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-RepoMan-RATA

Plasmid

#184270

Purpose

Expresses shRNA resistant GFP-RepoMan RATA Mutant

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell division cycle associated 2 (CDCA2 Human)

Tags

3xHA and GFP

Expression

Mammalian

Mutation

V383A, F395A

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-hWIPI2B-3×FLAG

Plasmid

#215510

Purpose

Expresses FLAG tagged human WIPI2B.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 2B (WIPI2 Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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FENEF-Sensor

Plasmid

#208930

Purpose

in vitro p38-Sensor for luminescent detection of p38-activity

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

FENEF sequence from human ATF2 with phospho site (ATF2 Human)

Tags

HISx6, MBP, and Smbit

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-EZH1-D745N

Plasmid

#203585

Purpose

Expresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.

Depositor

Abhishek Chakraborty, William Kaelin

Article

Chakraborty et al Sci Transl Med. 2017 Jul 12;9(398):eaal5

Insert

EZH1 (EZH1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

changed Aspartic Acid 745 to Asparagine for parti…

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ARID1A(42))-PGKpuro2ABFP-W

Plasmid

#200467

Purpose

Lentiviral vector expressing gRNA targeting human ARID1A

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ARID1A(42) (ARID1A Human)

Use

Lentiviral

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ARID1A(44))-PGKpuro2ABFP-W

Plasmid

#200468

Purpose

Lentiviral vector expressing gRNA targeting human ARID1A

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ARID1A(44) (ARID1A Human)

Use

Lentiviral

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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