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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,774 results for: BLI;

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Showing: 6961 - 6980 of 9774 results

  2. Hs.KRAS4a

    Plasmid
    #83166
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83166-plasmid-map-sequence-id-158129

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  3. pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50467
    Purpose
    Gi-coupled hM4D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has Service
    AAV2 and AAV8
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50467-plasmid-map-sequence-id-199198

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  4. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174138-plasmid-map-sequence-id-341915

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  5. Lamin A (R377H)-mRFP

    Plasmid
    #124270
    Purpose
    Lamin A mutant (R377H), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R377H) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R377H)
    Available Since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124270-plasmid-map-sequence-id-251113

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  6. pAAV-GFAP-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50471
    Purpose
    Gi-coupled hM4D-IRES-mCitrine under the control of GFAP promoter
    Depositor
    Bryan Roth
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50471-plasmid-map-sequence-id-75491

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  7. 6xO/S luc

    Plasmid
    #69445
    Purpose
    detection/assessment of OCT4 and SOX2 transcription factor activity
    Depositor
    Lisa Dailey
    Insert
    minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
    Use
    Luciferase
    Expression
    Mammalian
    Available Since
    May 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    69445-plasmid-map-sequence-id-259015

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  8. pIRESN2_GFP_YTA_Champ1_328-354

    Plasmid
    #105639
    Purpose
    Expresses fragment (amnio acids 328-354) of human Champ1 protein tagged with GFP
    Depositor
    Thanos Halazonetis
    Insert
    Champ1 (CHAMP1 Human)
    Tags
    GFP YTA
    Expression
    Mammalian
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105639-plasmid-map-sequence-id-203817

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  11. pAG416-GAL-FUS-G156E

    Plasmid
    #181716
    Purpose
    Galactose inducible expression of FUS-G156E
    Depositor
    Alejandro Chavez
    Insert
    FUS (FUS Human)
    Expression
    Yeast
    Mutation
    G156E
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181716-plasmid-map-sequence-id-430956

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  12. PRDX6A-c001

    Plasmid
    #175474
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    PRDX6 (PRDX6 Human)
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Mutation
    See Depositor Comments
    Available Since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    175474-plasmid-map-sequence-id-353175

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  13. pAAV-hSyn-GluA2-scFv-mNeon-KDEL

    Plasmid
    #230057
    Purpose
    AAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA2 single chain variable fragment (scFv)
    Use
    AAV
    Tags
    ER retrieval motif (KDEL) and mNeon
    Expression
    Mammalian
    Promoter
    human synapsin
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230057-plasmid-map-sequence-id-457175

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  14. HECTD1

    Plasmid
    #32860
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HECTD1 (HECTD1 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 1879-1966 only
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. SARS-CoV-2 Spike-d18

    Plasmid
    #164583
    Purpose
    A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.
    Depositor
    Jason Sheltzer
    Insert
    SARS-CoV-2 Spike (S SARS-CoV-2)
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1256-1273
    Promoter
    EF1a
    Available Since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    164583-plasmid-map-sequence-id-322362

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  16. SmBiT-b4GALT1 Fusion Vector

    Plasmid
    #237043
    Purpose
    Express SmBiT-b4GALT1 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    b4GALT1 (B4GALT1 Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237043-plasmid-map-sequence-id-471899

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  18. pALS3-Ma-sfGFP-TAG150

    Plasmid
    #212121
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212121-plasmid-map-sequence-id-444186

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  19. pBK-Ma pylRS nitroY/haloY-F5

    Plasmid
    #212125
    Purpose
    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.
    Depositor
    Ryan Mehl
    Insert
    M. alvus PylRS 3-NY/HaloY F5
    Expression
    Bacterial
    Mutation
    CTG125CTT, N166A V168S, A223C, W239R
    Promoter
    GlnS (constitutive)
    Available Since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    212125-plasmid-map-sequence-id-443626

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  20. KSR1-NanoLuc Fusion Vector

    Plasmid
    #237175
    Purpose
    Express KSR1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KSR1 (KSR1 Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237175-plasmid-map-sequence-id-473328

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Showing: 6961 - 6980 of 9774 results