We narrowed to 26,710 results for: SHE

1. PB-HD252

   Plasmid

   #172656

   Purpose

   Expressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 gene

   Depositor

   Jay Shendure

   Article

   Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   pegRNA-HD252A/pegRNA-HD252B
   

   Use

   CRISPR

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAGH50

   Plasmid

   #107254

   Purpose

   Vipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.

   Depositor

   Erin O'Shea

   Article

   Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

   Insert

   vipp1-gfp-fkbp12
   

   Use

   Suicide vector for destined for double homologous…

   Tags

   GFP and FKBP12

   Expression

   Bacterial

   Promoter

   native

   Available Since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. P0050::kaiBC S431A T432A NS1

   Plasmid

   #101837

   Purpose

   drives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locus

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   kaiBC S431A T432A with p0050 promoter
   

   Use

   Cyanobacteria cloning vector

   Mutation

   S431A T432A

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. P0050::kaiBC S431E T432E NS1

   Plasmid

   #101836

   Purpose

   drives expression of kaiBC S431E T432E variant from an rpaA-independent promoter in NS1 locus

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   kaiBC S431E T432E with p0050 promoter
   

   Use

   Cyanobacteria cloning vector

   Mutation

   S431E T432E

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRAF-MutL(R95F)

   Plasmid

   #86608

   Purpose

   E. coli Mutl(R95F) expression plasmid

   Depositor

   Richard Fishel

   Article

   Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

   Insert

   E. coli MutL(R95F)
   

   Tags

   his-tag and ald-tag

   Expression

   Bacterial

   Mutation

   R95F

   Promoter

   T7

   Available Since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSCKtheoRaj12

   Plasmid

   #69942

   Purpose

   pSTC2,TheoHHAzRaj12,cisRaj12-sfGFP, kanR

   Depositor

   Alfonso Jaramillo

   Article

   Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

   Insert

   TheoHHAzRaj12,cisRaj12,sfGFP
   

   Use

   Synthetic Biology

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSCKtheoRaj11

   Plasmid

   #69939

   Purpose

   pSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanR

   Depositor

   Alfonso Jaramillo

   Article

   Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

   Insert

   TheoHHAzRaj11,cisRaj11,sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCKbreak12

   Plasmid

   #69938

   Purpose

   pSTC2,cisRaj12-sfGFP, KanR

   Depositor

   Alfonso Jaramillo

   Article

   Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

   Insert

   CisRaj12-sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6_(GLuc)_INT[S1Apt]

   Plasmid

   #68425

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct with S1 streptavidin aptamer
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEBB-3XMyc-TRAF2 S408A

    Plasmid

    #44109

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 S408A (TRAF2 Human)
    

    Expression

    Mammalian

    Mutation

    Serine 408 to Alanine

    Available Since

    May 2, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pWB-TRAF2 S102A

    Plasmid

    #44135

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 S102A (TRAF2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Mutation

    Serine 102 to Alanine

    Available Since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pEBB-3XMyc-TRAF2 S102A

    Plasmid

    #44106

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 S102A (TRAF2 Human)
    

    Expression

    Mammalian

    Mutation

    Serine 102 to Alanine

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pCMV myc DAF-12(P687S)

    Plasmid

    #32567

    Depositor

    Al Fisher

    Article

    Hochbaum et al PLoS Genet. 2011 Jul;7(7):e1002179. Epub

    Insert

    DAF-12(P687S) (daf-12 Nematode)
    

    Tags

    myc epitodpe

    Expression

    Mammalian

    Mutation

    Proline 687 to Serine

    Promoter

    CMV

    Available Since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. human pirh2-(138-261)

    Plasmid

    #24882

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    Pirh2 (RCHY1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains aa 138-261.

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

15. human pirh2-(187-261)

    Plasmid

    #24883

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    Pirh2 (RCHY1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains aa 187-261.

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-hSyn-hM3D(Gq)-mCherry

    Plasmid

    #50474

    Purpose

    Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    hM3D(Gq)-mCherry (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-Bxb1o_nlsFlag

    Plasmid

    #119901

    Purpose

    Expresses Bxb1 integrase in mammalian cells

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    Mammalian codon optimized Bxb1 integrase
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX_311-KRAB-dCas9

    Plasmid

    #96918

    Purpose

    for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

    Depositor

    John Doench, William Hahn, David Root

    Article

    Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPTN-EGFP

    Plasmid

    #27052

    Depositor

    Beatrice Yue

    Article

    Park et al Am J Pathol. 2006 Dec . 169(6):1976-89.

    Insert

    optineurin (OPTN Human)
    

    Expression

    Mammalian

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmiRFP720-N1

    Plasmid

    #136560

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    miRFP720
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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