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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,957 results for: pET

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Showing: 6961 - 6980 of 9957 results

  1. 6D1T (MBD1)

    Plasmid
    #119966
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    MBD1 (MBD1 Human)
    Tags
    GSTtag, 6xHIS and Thrombin
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119966-plasmid-map-sequence-id-232690

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  2. Fast-GCaMP-EF16

    Plasmid
    #52642
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Publication
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-EF16
    Expression
    Mammalian
    Mutation
    D431N/D433N/D435N/E442A mutated from GCaMP3
    Promoter
    T7
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. TPK1

    Plasmid
    #32865
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TPK1 (TPK1 Human)
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. HERC2 (2KEO)

    Plasmid
    #25346
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    herc2.1203.1296 (HERC2 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 19, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. WDR5 (2H9L)

    Plasmid
    #25227
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5_01 (WDR5 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    lacking residues 1–23
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. PPI64

    Plasmid
    #25137
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ppi64.001.179 (PPIG Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    K125A/E126A
    Available since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. SRS-MTBD(19/19), AB(monomer), ux

    Plasmid
    #22389
    Depositor
    Ron Vale
    Insert
    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    Tags
    His and Myc
    Expression
    Bacterial
    Available since
    Nov. 24, 2009
    Availability
    Academic Institutions and Nonprofits only
    22389-plasmid-map-sequence-id-10296

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  9. SRS-MTBD(22/19), ux, dimer, no myc

    Plasmid
    #22390
    Depositor
    Ron Vale
    Insert
    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    Expression
    Bacterial
    Available since
    Nov. 24, 2009
    Availability
    Academic Institutions and Nonprofits only
    22390-plasmid-map-sequence-id-10297

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  13. His-AgdNK

    Plasmid
    #203226
    Purpose
    For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Publication
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
    Tags
    N-terminal TEV cleavable 6xHis tag
    Expression
    Bacterial
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203226-plasmid-map-sequence-id-403332

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  14. pTCF176 HLA-A*02:01 (CD8 mut)

    Plasmid
    #180459
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*02:01 (CD8 mut)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    D227K, T228A, replacement of transmembrane domain…
    Available since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    180459-plasmid-map-sequence-id-355125

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  15. FEM1C

    Plasmid
    #167842
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FEM1C (FEM1C Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 22, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167842-plasmid-map-sequence-id-331056

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  16. pKK-TEV-mEGFP

    Plasmid
    #105794
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mEGFP
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105794-plasmid-map-sequence-id-211381

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  17. pKK-TEV-mTagBFP

    Plasmid
    #105789
    Purpose
    Expression of your protein of interest in fusion with blue fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mTagBFP
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105789-plasmid-map-sequence-id-203380

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  18. 4YZ8

    Plasmid
    #69955
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WHSC1L1:JMC055-F10:C223308 (NSD3 Human)
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. BRPF3

    Plasmid
    #28269
    Depositor
    Cheryl Arrowsmith
    Insert
    BRPF3 (BRPF3 Human)
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains PWWP domain (residues 1056-1195).
    Available since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. PPIE

    Plasmid
    #25605
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PPIE (PPIE Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids 131-301.
    Available since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 6961 - 6980 of 9957 results