We narrowed to 4,243 results for: Pet2

1. pET28a Cdk2ap1ΔN (MT2B2)

   Plasmid

   #178034

   Purpose

   Expression vector for the purpose of protein purification.

   Depositor

   Lin He

   Article

   Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

   Insert

   Cdk2ap1 (Cdk2ap1 Mouse)
   

   Use

   Nonviral

   Tags

   6xHIS - Thrombin - MBP- TEV-TRS

   Expression

   Bacterial

   Mutation

   First N-Terminal 27aa are deleted

   Promoter

   T7LacI

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 6xHis-CPL2/pET28a

   Plasmid

   #172095

   Purpose

   cDNA of CER11/CPL2 cloned into pET28a for protein expression

   Depositor

   George Haughn

   Article

   Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

   Insert

   CPL2 (CPL2 Mustard Weed)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pET28_6xHis-PARP1delZn1-L713F

   Plasmid

   #173937

   Purpose

   Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn1-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 1-96; L713F, V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28_6xHis-PARP1delZn3-L713F

   Plasmid

   #173934

   Purpose

   Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn3-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 214-373; L713F, V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28_6xHis-PARP1-G871L

   Plasmid

   #174798

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-G871L (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G871L, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28_6xHis-PARP1-A870L

   Plasmid

   #174797

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A870L (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A870L, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28_6xHis-PARP1-K893I

   Plasmid

   #174799

   Purpose

   Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-K893I (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K893I, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21-pelB-LaG-43

   Plasmid

   #172759

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-43; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-43
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21-pelB-LaG-35

   Plasmid

   #172755

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-35; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-35
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21-pelB-LaG-19

    Plasmid

    #172748

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-19; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-19
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21-pelB-LaG-3

    Plasmid

    #172740

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-3
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21-pelB-LaG-12

    Plasmid

    #172744

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-12
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21-pelB-LaG-14

    Plasmid

    #172745

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-14
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-XPO5-His6

    Plasmid

    #171562

    Purpose

    Encodes human XPO5 protein

    Depositor

    Yan Zeng

    Insert

    XPO5 (XPO5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21a Ec-iPGM-bio

    Plasmid

    #162575

    Purpose

    Expresses E. coli iPGM containing a C-terminal biotinylation site and His tag

    Depositor

    James Inglese

    Article

    Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

    Insert

    E. coli iPGM bio-6His
    

    Use

    Tags

    6His tag, T7 tag, biotinylation sequence, and thr…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28_hTLN2-head-LBD

    Plasmid

    #164837

    Purpose

    Expression of human talin2 head mutant

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

    Insert

    TLN2 (TLN2 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Lipid binding deficient mutations

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28b-GFP-tglG

    Plasmid

    #134679

    Purpose

    Expresses His6-GFP-TglG fusion in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Insert

    TglG
    

    Use

    Tags

    His6-GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a SznF E281A

    Plasmid

    #139591

    Purpose

    expresses SznF E281A

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    SznF E281A
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    changed glutamate 281 to alanine

    Promoter

    T7

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a SznF H225A

    Plasmid

    #139590

    Purpose

    expresses SznF H225A

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    SznF H225A
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    changed histidine 225 to alanine

    Promoter

    T7

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-SznF E215A

    Plasmid

    #139565

    Purpose

    expresses SznF E215A in E. coli

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    SznF E215A
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    changed glutamate 215 to alanine

    Promoter

    T7

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-DDX43-NT-G46D

    Plasmid

    #134588

    Purpose

    Express human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coli

    Depositor

    Yuliang Wu

    Article

    Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

    Insert

    DDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    full-length (G154D)

    Promoter

    T7

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a-DDX43-FL-G84D

    Plasmid

    #134577

    Purpose

    Express human DDX43 mutant (full-length with G84D mutation) in E. coli

    Depositor

    Yuliang Wu

    Article

    Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

    Insert

    DDX43-G84D (DDX43 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    full-length (G84D)

    Promoter

    T7

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28B-PU.1-ETS-Wing

    Plasmid

    #124552

    Purpose

    Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    The 5 residues making up the "wing" in …

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28B-PU.1-ETS-Loop

    Plasmid

    #124551

    Purpose

    Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

    Depositor

    Gregory Poon

    Article

    Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

    Insert

    PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Residues 216 to 223 replaced with corresponding s…

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET21d-colEdes7/Imdes7

    Plasmid

    #107115

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des7

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes7/Imdes7
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21d-colEdes8/Imdes8

    Plasmid

    #107116

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des8

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes8/Imdes8
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET21d-colEdes9/Imdes9

    Plasmid

    #107117

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des9

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes9/Imdes9
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET21d-colEdes10/Imdes10

    Plasmid

    #107118

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des10

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes10/Imdes10
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET21d-colEdes11/Imdes11

    Plasmid

    #107119

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des11

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes11/Imdes11
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET21d-colEdes12/Imdes12

    Plasmid

    #107120

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des12

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes12/Imdes12
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET21d-colEdes13/Imdes13

    Plasmid

    #107121

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des13

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes13/Imdes13
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET21d-colEdes14/Imdes14

    Plasmid

    #107122

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des14

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes14/Imdes14
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET21d-colEdes15/Imdes15

    Plasmid

    #107123

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des15

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes15/Imdes15
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET21d-colEdes16/Imdes16

    Plasmid

    #107124

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des16

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes16/Imdes16
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET21d-colEdes17/Imdes17

    Plasmid

    #107125

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des17

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes17/Imdes17
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET21d-colEdes18/Imdes18

    Plasmid

    #107126

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des18

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes18/Imdes18
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET21d-colEdes1/Imdes1

    Plasmid

    #107108

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des1

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes1/Imdes1
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET21d-colEdes2/Imdes2

    Plasmid

    #107109

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des2

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes2/Imdes2
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET21d-colEdes3/Imdes3

    Plasmid

    #107110

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes3/Imdes3
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET21d-colEdes3.5/Imdes3.5

    Plasmid

    #107111

    Purpose

    plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3.5

    Depositor

    Sarel Fleishman

    Article

    Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

    Insert

    colEdes3.5/Imdes3.5
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge