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Showing: 681 - 700 of 10181 results

  3. pCJ207

    Plasmid
    #162680
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162680-plasmid-map-sequence-id-321455

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  7. pUDP044

    Plasmid
    #101168
    Purpose
    pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101168-plasmid-map-sequence-id-199309

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  14. pLCHKO_TK1_HPRT1_Lb_2

    Plasmid
    #155060
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and an intergenic site & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155060-plasmid-map-sequence-id-308523

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  16. pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD

    Plasmid
    #204359
    Purpose
    AAV vector for coexpression of miniDq and KORD under the control of human synapsin promoter
    Depositor
    Masao Doi
    Article
    Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008
    Insert
    miniDq-mCherry-P2A-HA-KORD
    Use
    AAV
    Tags
    HA (for KORD) and mCherry (for miniDq)
    Expression
    Mutation
    For miniDq, the third intracellular loop (ICL3) o…
    Promoter
    hSyn
    Available since
    Oct. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    204359-plasmid-map-sequence-id-401492

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Showing: 681 - 700 of 10181 results