We narrowed to 3,228 results for: Streptococcus
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Plasmid#135014PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptideDepositorInserthumanized S. pyogenes Cas9
Tags3x Flag, 3xFLAG (N terminal on insert), NLS, and …ExpressionMammalianMutation126aa domain from HSV-1 UL12 fused to the C-termi…PromoterCBhAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BsaI)_CBh-Cas9-T2A-mCherry
Plasmid#135012PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptideDepositorInserthumanized S. pyogenes Cas9
Tags3x Flag, 3xFLAG (N terminal on insert), NLS, and …ExpressionMammalianPromoterCBhAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLSB-HYG
Plasmid#166699PurposeCombined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
ExpressionYeastPromoteradh15 promoter and tRNA promoterAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHPHS800
Plasmid#228240PurposeBxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombinationDepositorInsertCas9
TagsHygromycin phosphotransferaseExpressionMammalianPromoternoneAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL.140
Plasmid#184995PurposeExpress Cas9 in human cellsDepositorInsertSpCas9
TagsSV40NLSExpressionMammalianPromoterTetOn-3gAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCXIN-EF1a-mNeonGreen-P2A-Cas9
Plasmid#189807PurposeRetroviral delivery of Cas9DepositorInsertCas9
UseRetroviralAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFREE2-SpCas9-sgRNA
Plasmid#179582PurposeExpresses spCas9 and gRNA that targets ColE1 plasmids for curingDepositorInsertCas9
ExpressionBacterialAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_EYFP
Plasmid#167858PurposePiggyBac compatible plasmid expressing spCas9DepositorInsertCas9
UseCRISPRAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-Cas9
Plasmid#166601PurposeEncodes Cre-dependent Cas9 under control of the TREDepositorInsertCas9
UseAAVPromotertetracycline response elementAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT-9218
Plasmid#124225PurposeBacterial SpCas9 expressionDepositorInsertSpCas9
UseCRISPRExpressionBacterialPromoterTetR/TetAAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX165-xCas9
Plasmid#140568PurposeExpresses xCas9 in mammalian cellsDepositorInsertCas9
MutationA262T R324L S409I E480K E543D M694I E1219VAvailable SinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK601
Plasmid#110607PurposeBTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)DepositorInsertCas9
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
px330 Rosa26 AscI LH291Gu1 (LH500)
Plasmid#99628PurposePlasmid used to target the pre-modified Rosa26 allele with Cas9DepositorInsertCas9
UseCRISPRTags3xFLAGAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHS248
Plasmid#87354PurposeS867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET constructDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/C574S/S867C/N1054CPromoterT7Available SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-SH3
Plasmid#79613PurposeExpresses dCas9-SH3 in yeast cellsDepositorInsertSpCas9
TagsSH3 domainExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1Available SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-NLS-3XHA-NLS
Plasmid#69220PurposeExpresses wild type SpCas9 in mammalian cellsDepositorInsertSpCas9
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268
Plasmid#69224PurposeExpresses R1335K mutant SpCas9 fused to Zif268 in mammalian cellDepositorInsertSpCas9
UseCRISPRTagsNLS-3XHA-NLS and Zif268ExpressionMammalianMutationArginine 1335 is changed to LysineAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag H588A
Plasmid#109032PurposeH588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag D16A+H588A
Plasmid#109033PurposeD16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
px459 VRER
Plasmid#101716PurposesgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)DepositorInsertSpCas9 VRER
Tags3xFLAG-NLS and NLSExpressionMammalianMutationVRER (D1135V, G1218R, R1335E and T1337R)Available SinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only