We narrowed to 14,460 results for: shi
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Plasmid#216489PurposeExpression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cellsDepositorInsertHaloAKAR2.1
TagsT2A-EGFPExpressionMammalianPromoterCMVAvailable SinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
enIscB
Plasmid#205410PurposeVector encoding human codon-optimized enhanced-activity OgeuIscB driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoterDepositorInsertpCAG_Flag_SV40NLS_OgeuIscB*_npNLS_polyA_pU6__RNA*_pCMV_mCherry
ExpressionMammalianMutationE85R+H369R+S387R+S457RPromoterCAG, hU6, CMVAvailable SinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T221A
Plasmid#108107PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP2
Plasmid#79874PurposeBacillus subtilis sgRNA expression vector; integrates into amyEDepositorInsertsgRNA RR1
UseCRISPRExpressionBacterialPromotervegAvailable SinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T142Q
Plasmid#108109PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & gatekeeper mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDOXOFF (STOP)99xGly
Plasmid#211349PurposeDOX-regulated lentiviral expression of 99xGly-EGFP-3xHA lacking initiation codonDepositorInsert(STOP)99xGly-EGFP-3xHA
UseLentiviralTagsEGFP-3xHAMutationStart (ATG) codon replaced with stop (TAG) codonAvailable SinceOct. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTagRFP658-N1
Plasmid#178974PurposeExpresses TagRFP658 in mammalian cellsDepositorInsertTagRFP658
TagsnoExpressionMammalianMutationNOPromoterCMVAvailable SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATG-3925 (CBG2)
Plasmid#167839PurposeExpresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cellsDepositorInsertClick Beetle Green 2
Tags6-HisExpressionBacterial and MammalianMutationQ348H. Click Beetle Green 2 (CBR2) has 3 amino a…PromoterCMV, T7Available SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP3
Plasmid#79875PurposeBacillus subtilis sgRNA expression vector; integrates into thrCDepositorInsertsgRNA RR1
UseCRISPRExpressionBacterialPromotervegAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
ATG-2460
Plasmid#108713PurposeEF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)DepositorInsertCBR2opt-copGFP fusion
UseLentiviralTagsCBR2opt fused to copGFPMutationPromega used directed evolution to create CBR (Cl…Promoterelongation factor-1Available SinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC94-pmax-dCas9VP160-2A-puro
Plasmid#48226PurposedCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.DepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
UseCRISPRTags2A-puro, HA Tag, and VP160ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
plentiCMV-sct-p5-b2m-HLA-A0101
Plasmid#196512PurposeExpress single-chain peptide-MHC (CMV p5 epitope on HLA-A0101)DepositorInsertsingle chain of a CMV peptide, b2m and HLA A0101
UseLentiviralTagsNAExpressionMammalianMutationY84CPromoterCMVAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3-P2A-GFP-PGK-Puro
Plasmid#110867PurposeLentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized)DepositorInsertBE3
UseLentiviralMutationD10APromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-ET
Plasmid#72542Purposeexpresses FLAG tagged human BRD4 ET domain (608-699 aa)DepositorAvailable SinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 HA-hTSPAN12
Plasmid#115784PurposeExpresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.DepositorInsertTSPAN12 (TSPAN12 Human)
TagsHAExpressionMammalianMutationmultiple silent mutations introduce restriction s…PromoterCMVAvailable SinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pphiLOV3-N1
Plasmid#178973PurposeExpresses phiLOV3 in mammalian cellsDepositorInsertphiLOV3
ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HaloAKAR2.1-CLC
Plasmid#216492PurposeExpression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) targeted to clathrin coated pitsDepositorInsertHaloAKAR2.1-CLC
ExpressionMammalianPromoterCMVAvailable SinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVU-coHPDL WT
Plasmid#174128PurposeExpresses wild type codon-optimized HPDL in mammalian cellsDepositorInsert4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
UseLentiviralTagsGFPExpressionMammalianMutationCodon optimizedAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-ET-F656W
Plasmid#72546Purposeexpresses FLAG tagged human BRD4 ET domain (608-699 aa) with F656W mutationDepositorAvailable SinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-ET-I654Q
Plasmid#72545Purposeexpresses FLAG tagged human BRD4 ET domain (608-699 aa) with I654Q mutationDepositorAvailable SinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only