We narrowed to 24,278 results for: pcdna

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pcDNA3.1-P2X2-CFP

Plasmid

#22401

Depositor

Henry Lester

Article

Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

Insert

ATP-gated P2X channel 2 (P2rx2 Rat)

Tags

CFP

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-Nsp7

Plasmid

#157688

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp7 (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5/FRT/TO DNAJA1

Plasmid

#19464

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJA1 (DNAJA1 Human)

Expression

Mammalian

Mutation

D244G

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-JNKAR1-NES

Plasmid

#84632

Purpose

Unimolecular, FRET-based JNK activity reporter; cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ECFP-FHA1-JNKsubDD-Citrine-NES

Tags

6xHis, Citrine, ECFP, Nuclear export signal (NES)…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pY019 (pcDNA3.1-hPmCpf1)

Plasmid

#69991

Purpose

Expresses humanized PmCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPmCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pY009 (pcDNA3.1-hSsCpf1)

Plasmid

#69981

Purpose

Expresses humanized SsCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hSsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-Rab10-FLAG

Plasmid

#135559

Purpose

Express mouse Rab10 in mammalian cells, FLAG at N terminal

Depositor

Jingshi Shen

Article

Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Rab10 (Rab10 Mouse)

Tags

Flag

Expression

Insect

Promoter

T7 promoter

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-AKARev(T/A)

Plasmid

#118479

Purpose

Negative-control mutant for RAB-AKARev biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-AKARev(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 SV8

Plasmid

#184011

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286).

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 (Prom1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Wild type cDNA

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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hSlo2.1 Slick/pCDNA3

Plasmid

#197317

Purpose

Plasmid expressing an antigen used to generate an antibody that targets the Slo2.1 Slick K+ channel subunit

Depositor

Christopher Lingle

Insert

Slo2.1 (Kcnt2 Mouse)

Tags

7xHis

Expression

Mammalian

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) Tacδ

Plasmid

#81177

Purpose

Mammalian expression of human Tacδ (GFFKR cytoplasmic tail)

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

Tacδ

Tags

KLGFFKR

Expression

Mammalian

Mutation

CD25 amino acids 1 to 263 fused to KLGFFKR cytopl…

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-C236A-pcDNA3.1

Plasmid

#85764

Purpose

mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236A

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)

Tags

HA

Expression

Mammalian

Mutation

catalytic inactive mutant C236A

Promoter

CMV

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-C236S-pcDNA3.1

Plasmid

#85763

Purpose

mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)

Tags

HA

Expression

Mammalian

Mutation

catalytic inactive mutant C236S

Promoter

CMV

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ Flag-RAGB99L

Plasmid

#128101

Purpose

Expresses constitutively GTP-bound RAGB in mammalian cells in a G418-selectable plasmid

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

Ras related GTP binding B (RRAGB Human)

Tags

Flag

Expression

Mammalian

Mutation

Q99L (CAA to CTA)

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-AktAR

Plasmid

#123356

Purpose

FRET-based biosensor for monitoring Akt activity in lipid raft plasma membrane regions.

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

Lyn-AktAR

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Paxillin/pcDNA3

Plasmid

#67775

Purpose

Paxilin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

Paxillin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-mCherry-N

Plasmid

#210271

Purpose

N-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Type

Empty backbone

Tags

mCherry

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Y587E EphA2 pcDNA3

Plasmid

#102730

Purpose

Expression of EphA2 in mammalian cells

Depositor

Jin Chen

Article

Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

Insert

EphA2 (Epha2 Mouse)

Expression

Mammalian

Mutation

Y587E (TAT > GAG)

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ROR1-V5/HIS

Plasmid

#205607

Purpose

Expression of human ROR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

ROR1 receptor tyrosine kinase (ROR1 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-hMcl-1(T163A)

Plasmid

#25376

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

hMCL1 (MCL1 Human)

Tags

V5 and His tag

Expression

Mammalian

Mutation

changed threonine 163 to alanine

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only