We narrowed to 15,981 results for: LEA

1. Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

   Plasmid

   #208969

   Purpose

   Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   EcKlenow-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV Flag dPDZ-PTEN (1-400)

   Plasmid

   #22232

   Depositor

   Hong Wu

   Article

   Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

   Insert

   PTEN (Pten Mouse)
   

   Tags

   Flag

   Expression

   Bacterial and Mammalian

   Mutation

   PDZ binding domain deleted. Contains amino acids …

   Available Since

   March 19, 2010

   Availability

   Academic Institutions and Nonprofits only

3. MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG

   Plasmid

   #163719

   Purpose

   Arabinose-inducible expression of six mutagenesis genes, each encoding one amber codon after the initiator methionine

   Depositor

   Matthew Bennett

   Article

   Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

   Insert

   araC dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TAG CDA1.1TAG
   

   Expression

   Bacterial

   Mutation

   dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TA…

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G

   Plasmid

   #146392

   Purpose

   Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.

   Depositor

   Elisa Izaurralde

   Article

   Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

   Insert

   DmeIF4G-trunc (eIF4G1 Fly)
   

   Expression

   Insect

   Mutation

   322-1666 truncated version of elF4G sequence with…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_NOPLight-ctr

   Plasmid

   #195579

   Purpose

   Expresses the control sensor NOPLight-ctr in mammalian cells

   Depositor

   Tommaso Patriarchi

   Article

   Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

   Insert

   NOPLight-ctr
   

   Expression

   Mammalian

   Mutation

   D110A, D130A

   Promoter

   CMV

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO_HA.NWS.Ollas_mCherry-NLS

   Plasmid

   #178263

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HA.NWS.Ollas and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMTB2-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80066

   Purpose

   Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Beta-actin2

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO_NWS.HA.V5_mCherry-NLS

   Plasmid

   #178283

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   NWS.HA.V5 and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST27-GST-hMSN

   Plasmid

   #211823

   Purpose

   Express human MSN in mammalian cells

   Depositor

   Haian Fu

   Article

   Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

   Insert

   MSN (MSN Human)
   

   Tags

   GST tag

   Expression

   Mammalian

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO_AU1.VSVg.V5_mCherry-NLS

    Plasmid

    #178236

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.VSVg.V5 and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO_AU1.S.VSVg_mCherry-NLS

    Plasmid

    #178234

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.S.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

    Plasmid

    #70668

    Purpose

    Human mutant of E2F1 lacking amino acids 2 to 126

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F1 lacking residues 2 to 126 (E2F1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    lacks amino acids 2-126

    Promoter

    cmv

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pAAV_hSynapsin1_NOPLight1

    Plasmid

    #195580

    Purpose

    Expresses NOPLight1 in neurons

    Depositor

    Tommaso Patriarchi

    Article

    Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

    Insert

    NOPLight1
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human Synapsin-1

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBactin-Nedd1-mNeonGreen

    Plasmid

    #196864

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mNeonGreen (Nedd1 Rat)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-TRE-HTR6-SNAPf-TRIP

    Plasmid

    #189617

    Purpose

    Expresses cilia-targeted RhoA inhibitory peptide, tet-inducible

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    HTR6-TRIP_alpha
    

    Use

    AAV

    Tags

    alphaTag in the N-term of HTR6, SNAP-tag in the C…

    Promoter

    TRE

    Available Since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. OCT4 GFP puro Donor 2

    Plasmid

    #22210

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    SA-OCT-GFP-2APuro-PA (POU5F1 Human)
    

    Available Since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO_C.HA.V5_mCherry-NLS

    Plasmid

    #178242

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HA.V5 and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a_DIO_R106W-HaloTag

    Plasmid

    #164063

    Purpose

    Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R106W mutation

    Depositor

    Nathaniel Heintz

    Article

    Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

    Insert

    MeCP2 R106W (Mecp2 Mouse)
    

    Use

    AAV

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7-EGFP-C1-HsNot1iso1-V1251R_R

    Plasmid

    #147376

    Purpose

    Mammalian Expression of HsNot1iso1-V1251R

    Depositor

    Elisa Izaurralde

    Article

    Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

    Insert

    HsNot1iso1-V1251R (CNOT1 Human)
    

    Expression

    Mammalian

    Mutation

    A deletion of 5 AA 822-827 SKMKPS-> T and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. VP16-ER beta (short)

    Plasmid

    #11353

    Depositor

    Donald McDonnell

    Article

    Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

    Insert

    ER beta short (ESR2 Human)
    

    Expression

    Mammalian

    Available Since

    March 10, 2006

    Availability

    Academic Institutions and Nonprofits only