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pET23a_mod_RBPMS2_FL

Plasmid

#59394

Purpose

Plasmid for bacterial overexpression of RBPMS2

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS2 (RBPMS2 Human)

Tags

6xHis, FLAG, HA, and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5-rat ERK2-L73P

Plasmid

#40817

Depositor

Natalie Ahn

Article

Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

Insert

MAPK1 (Mapk1 Rat)

Tags

His6

Expression

Mammalian

Mutation

L73P

Promoter

CMV

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pISH-Gnas

Plasmid

#105483

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Gnas (Gnas Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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HOXD12 (murine) HIS-tag pET

Plasmid

#8553

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXD12 (Hoxd12 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pCRII-Topo U6-SpAi9L-U6SpDMDL

Plasmid

#78608

Purpose

Expresses gRNAs (for SpCas9) targeting 5’ of Ai9 stop cassette and Dmd intron 22

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SpAi9L-U6SpDMDL

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only
pCRII-Topo U6-SpAi9R-U6-SpDMDR

Plasmid

#78609

Purpose

Expresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SpAi9R-U6-SpDMDR

Use

Unspecified

Promoter

U6

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ADAM7 (wt)

Plasmid

#31139

Depositor

Yardena Samuels

Article

Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

Insert

ADAM7 (ADAM7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

wt

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ERBB4 (E542K)

Plasmid

#29530

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed glutamic acid 542 to lysine

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pLKO-Cre sgAdam10 v3

Plasmid

#158034

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Adam10 sgRNA

Expression

Mammalian

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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mIFP-PDHA1-N-10

Plasmid

#56236

Purpose

Localization: Mitochondrial Matrix, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

PDHA1 (PDHA1 Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pet28a_RBPMS_1_129

Plasmid

#65738

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-129

Promoter

T7

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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psiCheck-ApoE3'UTR

Plasmid

#41846

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

Insert

ApoE 3'UTR (APOE Human)

Use

Luciferase

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
2XMyc-LRRK2-RCK-K1347A

Plasmid

#25066

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the ROC-COR-Kinase domains aa 1329…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pAS19

Plasmid

#158788

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans ASH neurons

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

psra-6::GCaMP6m::rpl-1a

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAS16

Plasmid

#158786

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Pntc-1::GCaMP6m::rpl-1a

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB4 (E563K)

Plasmid

#29532

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed glutamic acid 563 to lysine

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only
MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-GFP

Plasmid

#75130

Purpose

Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
LP454

Plasmid

#90185

Purpose

pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 1-649

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

NPHP4 (NPHP4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

aa residues 1-649

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_1_100

Plasmid

#65735

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-100

Promoter

T7

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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p3E-Dnm2a-DN

Plasmid

#109572

Purpose

Multisite gateway vector for 3' tagging with dominant negative Dynamin2a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dynamin2a (dnm2a Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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