We narrowed to 18,348 results for: multi
-
Plasmid#137873PurposePiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTCF4 (TCF4 Human)
UseCRISPRExpressionMammalianAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2K-LRL-mouse c-MYC-IRES-Luc2
Plasmid#109228PurposeFor Tol2 transposon-mediated stable expression of LRL, c-Myc and IRES-luciferaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertc-Myc (Myc Mouse)
TagsIRES-Luc2ExpressionMammalianPromoterCAGGSAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1(+)-HA-DLC1-i4
Plasmid#99534PurposeDLC1-isoform 4 expression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDLC1-isoform 4 (DLC1 Human)
TagsHAExpressionMammalianPromoterCMVAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYpet-Optop38i
Plasmid#89749PurposeExpression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13FDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOptop38i3
UseFluorescent proteinTagsYpetExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A
Plasmid#51257PurposeEncodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to AlaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGli2 (Gli2 Mouse)
UseFlpin systemTags3xHA-TEVExpressionMammalianMutationamino acids 216,220,224,230,248,789,805,817,848,9…PromoterEF1aAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFIN-EF1a-GFP:2A:CHER(HA)-WPRE
Plasmid#44597DepositorInsertEF1a-GFP:2A:CHER(HA)
UseLentiviralPromoterhuman elongation factor -1a (pos 2528-3971)Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron-T
Plasmid#36885DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsGFP
tdTomato-3Myc
beta-globin intron
Tags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GPER-DuET
Plasmid#213256PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPER (GPER1 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA
Plasmid#210501Purposeexpresses LAUNCHER component in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA
TagsMycExpressionMammalianPromoterCMVAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
Plasmid#176624PurposeTF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3DepositorInsertTRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
UseSynthetic BiologyAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
DHC1-AID-Clover donor (Neo)
Plasmid#158621PurposeDonor plasmid for tagging DHC1 with AID-CloverDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDHC1 (DYNC1H1 Human)
UseCRISPR; Tagging donorAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Pard3-EGFP-PIF6
Plasmid#154914PurposeFull length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpar-3 family cell polarity regulator alpha, b (pard3ab Zebrafish)
UseXenopus/avian/zebrafishTags1-100 amino acids of Arabidopsis PIF6 and EGFPExpressionMammalianPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {mCAR}off-{ChETA-mRuby2}on-W3SL
Plasmid#111390PurposeAAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsUseAAVTagsMyc and mRuby2ExpressionMammalianPromoterhSynapsinAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {hCAR}off-{ChETA-EYFP}on-W3SL
Plasmid#111392PurposeAAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON ChETA-EYFP (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsUseAAVTagsEYFP and MycExpressionMammalianPromoterhSynapsinAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
G-GECO1.2-Orai1Y80E
Plasmid#73565PurposeGreen fluorescent reporter of mutant Orai1Y80E-associated calcium influxDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertG-GECO1.2-Orai1Y80E (ORAI1 Synthetic, Human)
TagsG-GECO1.2ExpressionMammalianMutationchanged tyrosine 80 of Orai1 to glutamatePromoterCMV Immediate EarlyAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGGD_MiniTurboID-YFP
Plasmid#222434PurposeGolden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMiniTurboID (BirA mutant)
UseGolden gate / green gate compatible cloning vectorTagsLinker and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MF85_PB-14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
Plasmid#176629PurposeTF C self-activation cassette in MultiFate-3DepositorInsert14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
UseSynthetic BiologyAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-tetpA(hBCL2-miR9/9*-124)-iRPT
Plasmid#154881PurposeDoxycycline-dependently expresses human BCL2 and murine miR9/9*-124. Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsExpressionMammalianAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394
Plasmid#128387PurposeExpresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruptionDepositorUseLentiviral, Luciferase, and Synthetic BiologyTagsCLuc394 and NLuc398ExpressionMammalianPromoterEFS and IRESAvailable sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUG-amdSYM-mak10TR
Plasmid#75433PurposeGenerates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiaeDepositorInsertsMAK10TR
ApR
amdSYM
ExpressionYeastMutationN-terminal 740 bp of MAK10 ORFPromoterNative promoter and PTEFAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only