We narrowed to 92,501 results for: lias

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Lyn-CFP-GAI(1-151)

Plasmid

#37312

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

CFP and Lyn

Expression

Mammalian

Mutation

contains aa 1-151

Promoter

CMV

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
pUASp-FMW-attB-AthHen1

Plasmid

#104957

Purpose

Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila germline tissues using Gal4 drivers

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Tags

FLAG Myc

Expression

Insect

Promoter

pTrasposase promoter

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV CreERT2 L5-L2

Plasmid

#62172

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and CreERT2 module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

CreERT2

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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GFP Snail 4SE

Plasmid

#16230

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

4SE: S108E, S112E, S116E, S120E

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR CMV PuroR L3-L2

Plasmid

#62163

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and Puromycin module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Puromycin

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf76

Plasmid

#12764

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf76

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

Plasmid

#127308

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV10/3xflag-Clock L57E

Plasmid

#47337

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

L57E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127316

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-casp9_casp9-AtCRY2-PHR

Plasmid

#131223

Purpose

Opto-casp9_casp9-AtCRY2-PHR (light-activated caspase9)

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

Opto-casp9 (casp9-AtCRY2-PHR)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV10/3xflag-Clock L74E

Plasmid

#47338

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

L74E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pBW1310_pCAG-PV1-NTD-ENTRY-L1-PYL-NLS-BGHpA

Plasmid

#108838

Purpose

Insert protein fragment into MluI/BspEI to fuse to PYL/NLS tags on C-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

PYL,NLS

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpA

Plasmid

#108839

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to ABI tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

ABI

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW1049_pCAG-PV1-NTD-ENTRY-L1-FKBP-NLS-BGHpA

Plasmid

#108834

Purpose

Insert protein fragment into MluI/BspEI to fuse to FKBP/NLS tags on C-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

FKBP,NLS

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW1050_pCAG-PV1-NLS-FRB-L1-CTD-ENTRY-BGHpA

Plasmid

#108835

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to FRB/NLS tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6 stuffer sgRNA scaffold L5-L2

Plasmid

#62130

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

CRISPR; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6 n.s shRNA L5-L4

Plasmid

#62138

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and a non-silencing (scrambled) shRNA module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

non-silencing (scrambled) shRNA

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Promoter

U6

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-FLJ23445

Plasmid

#15429

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

FLJ23445 (Thoc7 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf98

Plasmid

#12786

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf98

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR CMV eGFP L5-L2

Plasmid

#62140

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

eGFP

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf78

Plasmid

#12766

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf78

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127318

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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LZRS ATG12 ires zeo

Plasmid

#67531

Purpose

For retroviral based stable expression of human ATG12

Depositor

Stephen Tait

Article

Haller et al Autophagy. 2014;10(12):2269-78. doi: 10.

Insert

APG12 (ATG12 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR SV40 iRFP L3-L2

Plasmid

#62160

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and iRFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

iRFP

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

SV40

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pPK-351

Plasmid

#157921

Purpose

pcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBD

Depositor

Phillip Kyriakakis

Article

Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

Inserts

PIF3-MTAD
IRES
PhyB(1-621)-SV40NLS-Gal4DBD

Use

Synthetic Biology; Optogenetics

Tags

HA tag and SV40NLS

Expression

Mammalian

Mutation

pif3 1-523

Promoter

CMV

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-HA-UFL1-RAMP4

Plasmid

#139863

Purpose

Lentiviral expression of fusion of human UFL1 (full length) with RAMP4

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

UFL1-RAMP4 (UFL1 Human)

Use

Lentiviral

Tags

HA

Mutation

recombinant fusion of UFL1 (full length) with RAM…

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBCMV-MS2CP-eDHFR

Plasmid

#167309

Purpose

Template pDNA to prepare a split CaVT component mRNA

Depositor

Hirohide Saito

Article

Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

Insert

MS2CP-eDHFR

Use

Synthetic Biology

Tags

DYK-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV eGFP R4-R3

Plasmid

#62141

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and EGFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

eGFP

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-dLbCpf1-BE0ΔiNLS

Plasmid

#107684

Purpose

Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE0ΔiNLS

Expression

Mammalian

Mutation

D832A/E925A/D1148A

Promoter

CMV

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pFUGW-GFP-2-cut reporter

Plasmid

#131669

Purpose

Lentiviral plasmid that expresses a GFP-based reporter for monitoring CRISPR based precise end-joining.

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

GFP-2-cut reporter

Use

Lentiviral

Tags

P2A

Expression

Mammalian

Promoter

EFS-NS

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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